黄瓜种子萌发对NO_3^-胁迫的响应及耐盐性评价  

Response and Stress Tolerance Evaluation of Different Cucumber Cultivars under NO_3^- Stress

作　　者：徐慧妮[1,2] 王秀峰[1] 孙旭东[1] 杨凤娟[1] 史庆华[1] 

机构地区：[1]作物生物学国家重点实验室山东农业大学园艺科学与工程学院,山东泰安271018 [2]昆明理工大学生命科学与技术学院,昆明650224

出　　处：《西北植物学报》2011年第2期325-331,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30471187)

摘　　要：通过培养皿发芽试验,研究了0、14、98、182 mmol/L NO3-对7个黄瓜品种种子萌发的影响,并用隶属函数法对它们的耐盐性进行综合评价.结果表明:与对照相比较,14 mmol/L NO3-处理使7个黄瓜品种种子的相对发芽率、相对发芽指数、耐盐指数9、6 h后的鲜重、干重和超微弱发光(UWL)强度均增加,而98、182 mmol/L NO3-处理下这些指标均逐渐降低;各幼苗的电解质相对渗漏率在14 mmol/L NO3-处理后降低,而在98、182 mmol/LNO3-处理后其值逐渐增加;随着NO3-浓度的增加,各黄瓜品种的胚根总面积、总长度逐渐减少.各黄瓜品种半致死浓度值(LC50)以津园5号的最大(192.3 mmol/L),新秀4号的最小(105.0 mmol/L).不同黄瓜品种耐盐性的综合评价显示,津园5号、新泰密刺为耐盐品种,新秀4号、神农春五为盐敏感品种,其他为中度耐盐品种.The effects of 0,14,98,and 182 mmol/L NO-3 on germination of 7 different cucumber cultivars in Petri dishes were studied and the NO-3 stress tolerance of different cucumber cultivars was evaluated.The results indicated that the relative germination rate,relative germination index,salt stress index,fresh weight,dry weight and ultraweak luminescence（UWL） intensity were increased after 14 mmol/L NO-3 treatment,but decreased after 98 and 182 mmol/L NO-3 treatments.The relative electrolyte leakage decreased after 14 mmol/L NO-3 treatment,but increased with 98 and 182 mmol/L NO-3 treatments.The total area and total length of radicals were decreased with the increase of NO-3 concentration.The value of LC50 of ＇Jinyuan No.5＇ and ＇Xinxiu No.4＇ were 192.3 mmol/L,105.0 mmol/L,respectively.In all of the 7 cultivars,＇Jinyuan No.5＇ and ＇Xintaimici＇ were salt tolerant cultivars,while ＇Xinxiu No.4＇ and ＇Shennongchunwu＇ were salt sensitive cultivars and others were intermediate salt tolerance by subordinate function values analysis.

关 键 词：黄瓜 NO3-胁迫 超微弱发光 LC50 隶属函数 

分 类 号：Q945.35[生物学—植物学]