不同培养条件对铁线莲不定芽增殖及玻璃化的影响  

Effect of Culture Condition on the Multiplication and Vitrification of Shoots Culture for Clematis 'Multi-Blue'

作　　者：袁佳[1] 胡恒康[2] 方炎明[3] 张启香[1] 

机构地区：[1]浙江农林大学林业与生物技术学院,浙江临安311300 [2]江西农业大学园林与艺术学院,南昌330045 [3]南京林业大学森林资源与环境学院,南京210037

出　　处：《西北植物学报》2011年第2期401-406,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：浙江农林大学博士启动基金(2008FR021)

摘　　要：以铁线莲品种Multi-Blue萌发的新芽为外植体,从植物生长调节物质(BA和NAA)及转接周期等方面,探讨控制或减轻铁线莲Multi-Blue组培苗玻璃化的途径.结果表明:(1)接种在含MS+1.0 mg·L-1BA+0.1 mg·L-1NAA培养基中的Multi-Blue不定芽有效增殖系数最高,达4.18,显著高于其他处理;BA/NAA相对比值对不定芽有效增殖率无显著性影响;铁线莲Multi-Blue继代培养的最佳周期为14 d.(2)叶片超微结构观察发现,铁线莲Multi-Blue玻璃化苗叶肉细胞中的叶绿体膨胀,体积增大,形状不规则;类囊体片层或消失或变得模糊不清;嗜锇滴体积增大,细胞核固缩.New shoots of Clematis ＇Multi-Blue＇ were used as the explants,and effects of the plant growth regulators（BA and NAA） and switching time during shoot multiplication of Clematis ＇Multi-Blue＇ were comparatively analyzed to control vitrification.Also ultrastructure change of vitrifiable leaves was observed.Effective multiplication rate of shoots inoculated in MS medium supplemented with 1.0 mg·L-1 BA and 0.1 mg·L-1 NAA was significantly higher,with a 4.18,than that in other treatments.There has no significantly difference in shoot multiplication in relative value of BA and NAA.It was suggested that the optimal switching time for vitrification control was at 14 days interval.The observation result of ultrastructure showed that the chloroplasts in mesophyll cells of vitrifiable leaves swelled.The shape of the chloroplasts in mesophyll cells of vitrificated leaves was irregular.Thylakoid lamella disappeared or turned blurry.Osmiophilic droplet augmented and the nucleolus shrinked.

关 键 词：铁线莲Multi-Blue 植物生长调节物质 继代周期 玻璃化现象 超微结构 

分 类 号：Q813.1[生物学—生物工程]