氯化锰标记前列腺癌细胞株的体外MRI研究  

Magnetic resonance imaging of prostate cancer cell lines labled with manganese chloride in vitro

作　　者：庄文权[1] 范惠双[2] 张小玲[1] 向贤宏[1] 唐欲博[3] 毛丽娟[1] 邹学农[4] 

机构地区：[1]中山大学附属第一医院医学影像科,广州510080 [2]东莞市人民医院介入科,广东523018 [3]中山大学附属第一医院 药学部,广州510080 [4]中山大学附属第一医院 骨科,广州510080

出　　处：《影像诊断与介入放射学》2011年第1期10-14,共5页Diagnostic Imaging & Interventional Radiology

基　　金：广东省科技计划项目(2008B030301049);教育部博士点新教师基金(200805581170)

摘　　要：目的 初步探索使用氯化锰（MnCl2）标记前列腺癌细胞株（PC-3）行体外MRI的可行性和安全性.方法 将PC-3进行复苏、培养和扩增.在细胞培养箱中,PC-3与8组含不同MnCl2浓度的F-12 HAM＇S培养基共同培养1h,收集已标记的PC-3并行MRI.另对不同细胞数量级和不同时间点的MnCl2标记的PC-3行MRI.用含维拉帕米的培养基培养MnCl2标记的PC-3 4 h,在不同时间点取标记后的PC-3行MRI.用WST-8法测定氯化锰标记的PC-3活性.结果 MnCl2标记的PC-3在T1WI显示为高信号,其信号强度与未标记的PC-3形成明显差异（P＜0.01）,以1.0 mmol /L MnCl2为标记浓度时信号强度最强.1.0mmol/L MnCl2标记的PC-3在细胞数量为0.5×106时可呈高信号.在体外培养的条件下,标记后24 h的PC-3信号强度明显下降,标记后72 h基本恢复至未标记的PC-3信号强度水平.维拉帕米可延长MnCl2标记的PC-3有效成像时间至72 h.标记后4 h,除0.1 mmol/L MnCl2对PC-3的活性没有影响（P ＞ 0.05）外,其余各浓度组（＞ 0.1mmol/L）的MnCl2对PC-3的活性均有不同程度的影响（P ＜ 0.05）;标记后24h,0.5 ～ 1.0 mmol/L MnCl2对细胞的活性影响不明显（P ＞ 0.05）.结论 MnCl2可以有效标记PC-3,并能在T1WI以高信号成像且具有一定灵敏度.在浓度低于或等于1.0 mmol/L时,标记PC-3有一定的安全性,但标记维持时间较短.钙离子通道阻滞剂（维拉帕米）可适当延长PC-3的MnCl2标记维持时间.Objective To assess the feasibility and security of prostate cancer cell lines （PC-3） labeled with manganese chloride （MnC12） for magnetic resonance imaging（MRI） in vitro. Methods The PC-3 that purchased from American Type Culture Collection（ATCC） were recovered, cuhured and amplified. The PC-3 were cultured in F-12 HAM＇S medium with different concen- trations of MnC12 in cell incubator and collected for MRI after 1 hour. The labeled ceils were also collected for MRI in different amount and different time after labeling. The labeled cells were incubated with verapami! for 4 hours and the changes of the labeled cellular signal intensities were recorded in different time. Cell Counting Kit-8 （CCK-8） was used to determine the activities of the labeled cells. Results The PC-3 labeled with MnC12 were high signal intensities on Tl-weighted MRI. There were statistically sig- nificant differences between labeled cells and unlabeled cells （P〈 0.01）. Highest signal intensity reached when PC-3 were labeled with 1.0 mM MnC12. The signal intensity obviously decreased after 24 hours and became to normal signal intensity of unlabded PC-3 after 72 hours. The PC-3 labeled with 1.0 mM MnC12 solution showed high signal intensity on Tl-weighted MRI with the min- imum cell amount of 5.0 x 105 and lasted to 72 hours after a 4 hours incubation with verapamil. After 4 hours labeling, except the concentration of 0.1 mM, the other concentrations of MnC12 （ 〉0. lmM） had a certain toxicity on PC-3 （P〈O.05）. But, after 24 hours, the concentrations ofO.5-1.O mM had little effect on the viability of PC-3 （P〉O.05）. Conclusion The PC-3 could be la- beled with MnC12 and appears high signal intensity on Tl-weighted MRI. The PC-3 can be safety labeled with MnC12 in concentrations which were equal or less than 1.0 raM, but the duration of Mn＋2 in PC-3 is shorter. Calcium channel blocker （verapamil） may be extend the duration of PC-3 labeled with MnC12.

关 键 词：锰 离子 前列腺癌细胞株 细胞标记 磁共振成像 

分 类 号：R445.2[医药卫生—影像医学与核医学] R737.25[医药卫生—诊断学]