Phylogenetic Relationships of 3/3 and 2/2 Hemoglobins in Archaeplastida Genomes to Bacterial and Other Eukaryote Hemoglobins  

作　　者：Serge N. Vinogradov Ivan Fernandez David Hoogewijs Rail Arredondo-Peter 

机构地区：[1]Department of Biochemistry and Molecular Biology, Wayne State University, School of Medicine, Detroit, MI 48201, USA [2]Laboratorio de Biofisica y Biologia Molecular, Facultad de Ciencias, Universidad Autonoma del Estado de Morelos, 62210 Cuernavaca, Morelos, Mexico [3]Institute of Physiology and Zurich Center for Integrative Human Physiology (ZIHP), University of Zurich, Zurich, Switzerland

出　　处：《Molecular Plant》2011年第1期42-58,共17页分子植物（英文版）

摘　　要：Land plants and algae form a supergroup, the Archaeplastida, believed to be monophyletic. We report the results of an analysis of the phylogeny of putative globins in the currently available genomes to bacterial and other eu- karyote hemoglobins （Hbs）. Archaeplastida genomes have 3/3 and 2/2 Hbs, with the land plant genomes having group 2 2/ 2 Hbs, except for the unexpected occurrence of two group 1 2/2 Hbs in Ricinus communis. Bayesian analysis shows that plant 3/3 Hbs are related to vertebrate neuroglobins and bacterial flavohemoglobins （FHbs）. We sought to define the bacterial groups, whose ancestors shared the precursors of Archaeplastida Hbs, via Bayesian and neighbor-joining anal- yses based on COBALTalignment of representative sets of bacterial 3/3 FHb-like globins and group I and 2 2/2 Hbs with the corresponding Archaeplastida Hbs. The results suggest that the Archaeplastida 3/3 and group 1 2/2 Hbs could have orig- inated from the horizontal gene transfers （HGTs） that accompanied the two generally accepted endosymbioses of a pro- teobacterium and a cyanobacterium with a eukaryote ancestor. In contrast, the origin of the group 2 212 Hbs unexpectedly appears to involve HGT from a bacterium ancestral to Chloroflexi, Deinococcales, Bacilli, and Actinomycetes. Furthermore, although intron positions and phases are mostly conserved among the land plant 3/3 and 2/2 globin genes, introns are absent in the algal 3/3 genes and intron positions and phases are highly variable in their 2/2 genes. Thus, introns are irrelevant to globin evolution in Archaeplastida.Land plants and algae form a supergroup, the Archaeplastida, believed to be monophyletic. We report the results of an analysis of the phylogeny of putative globins in the currently available genomes to bacterial and other eu- karyote hemoglobins （Hbs）. Archaeplastida genomes have 3/3 and 2/2 Hbs, with the land plant genomes having group 2 2/ 2 Hbs, except for the unexpected occurrence of two group 1 2/2 Hbs in Ricinus communis. Bayesian analysis shows that plant 3/3 Hbs are related to vertebrate neuroglobins and bacterial flavohemoglobins （FHbs）. We sought to define the bacterial groups, whose ancestors shared the precursors of Archaeplastida Hbs, via Bayesian and neighbor-joining anal- yses based on COBALTalignment of representative sets of bacterial 3/3 FHb-like globins and group I and 2 2/2 Hbs with the corresponding Archaeplastida Hbs. The results suggest that the Archaeplastida 3/3 and group 1 2/2 Hbs could have orig- inated from the horizontal gene transfers （HGTs） that accompanied the two generally accepted endosymbioses of a pro- teobacterium and a cyanobacterium with a eukaryote ancestor. In contrast, the origin of the group 2 212 Hbs unexpectedly appears to involve HGT from a bacterium ancestral to Chloroflexi, Deinococcales, Bacilli, and Actinomycetes. Furthermore, although intron positions and phases are mostly conserved among the land plant 3/3 and 2/2 globin genes, introns are absent in the algal 3/3 genes and intron positions and phases are highly variable in their 2/2 genes. Thus, introns are irrelevant to globin evolution in Archaeplastida.

关 键 词：Algae Archaeplastida evolution HEMOGLOBIN land plants truncated Hbs 

分 类 号：Q51[生物学—生物化学] S852.612[农业科学—基础兽医学]