慢性乙型肝炎HBsAg，HBeAg和DNA变化的相关性研究  

The relationship of serum HBsAg, HBeAg concentration and HBV-DNA load in chronic hepatitis B during IFN treatment

作　　者：李明慧[1] 谢尧[1] 邱国华[1] 路遥[1] 赵辉[1] 杨民[1] 宋淑静[1] 刘凤[1] 张书凤[1] 陈利军[1] 成军[1] 徐道振[1] 

机构地区：[1]首都医科大学附属北京地坛医院肝病中心,北京100015

出　　处：《中华实验和临床病毒学杂志》2011年第1期26-28,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的探讨HBeAg阳性慢性乙型肝炎IFN-a治疗中HBsAg、HBeAg和HBV—DNA含量变化的相关性。方法对HBeAg阳性慢性乙型患者，在人组后进行IFN-a3MU-5MU隔日皮下注射一次的抗病毒治疗，于治疗前和治疗中的每间隔3个月收集血清，用实时荧光定量PCR进行HBV-DNA含量检测，血清HBsAg、HBeAg含量采用美国Abbott微粒化学发光法检测试剂，ARCHITECHi2000检测。HBV-DNA含量的检测下线为5×10^2拷贝／ml。分析IFN治疗过程中血清HBV—DNA、HBeAg和HBsAg含量变化的相关性。结果人组患者228例，男性162例，女性66例，年龄14～60岁，平均30．94岁；治疗过程中HBV—DNA、HBeAg和HBsAg的含量均逐渐下降干扰素治疗前以及治疗期间HBsAg与HBeAg、HBsAg与HBV—DNA含量变化间无相关性（P〉0．05）；而血清HBeAg与HBV—DNA含量变化相关性有统计学意义（P〈0．05）；随着干扰素治疗时间的延长，HBeAg与HBV—DNA含量逐渐下降，二者变化趋势一致。结论干扰素治疗前及治疗期间，HBeAg与HBV—DNA含量变化密切相关，而HBsAg含量变化与HBeAg和HBV—DNA含量变化无相关性，干扰素治疗期间需对三指标同时检测。Objective To investigate the relation of serum HBsAg, HBeAg contents and HBV-DNA load changes in HBeAg-positive chronic hepatitis B during IFN-a treatment. Methods After enrolled, the patients were treated with 3MU-SMU IFN subcutaneous injection every two days, and their serum was collected before treatment and every 3 months during the treatment course. The serum HBV-DNA load was determined by real-time fluorescence quantitative PCR method kit （ lower detection limit 500 copies/ml, Piji company, Shenzhen city, China,） according to production instruction, and HBeAg and HBsAg contents were detected by ARCHITECH 1 2000.chemiluminescent kit. The relation of.serum HBV-DNA, HBeAg and HBsAg content was analyzed by SPSS statistic software. Results There were 228 patients enrolled into this group, male 162 cases, female 66 cases, aged 14-60 years, average 30. 94 years old. After IFN treatment the HBV-DNA, HBeAg and HBsAg levels were all gradually decreased. But there was no relation of HBsAg content to HBV DNA and HBeAg content before and during treatment course（ P 〉 0.05 ）. However the serum HBeAg content was related to HBV-DNA content significantly （P 〈 0. 05 ） and their Changes was correspondence. Conclusion Before and during treatment of interferon, HBeAg and HBV-DNA content changes are closely related, while there is no significant correlation between HBsAg and HBeAg and HBV- DNA content. During interferon therapy, HBsAg, HBeAg, HBV-DNA contents should be detected together.

关 键 词：肝炎 乙型 慢性 治疗结果 干扰素-A 肝炎表面抗原 乙型 肝炎e抗原  乙型 DNA 

分 类 号：R512.62[医药卫生—内科学]