小鼠早期胚胎质量与细胞凋亡的相关性  

Association between cell apoptosis and the quality of early mouse embryos

作　　者：余敏[1,2] 邱卓琳[1] 李红[1] 曾位森[3] 陈雷宁[1] 李秋华[1] 全松[1] 

机构地区：[1]南方医科大学南方医院妇产科生殖医学中心,广东广州510515 [2]江西省妇幼保健院辅助生殖中心,江西南昌330006 [3]南方医科大学细胞生物学教研室,广东广州510515

出　　处：《南方医科大学学报》2011年第3期409-413,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(30872769)~~

摘　　要：目的分析小鼠早期胚胎质量与细胞凋亡的关系,了解细胞凋亡调控基因在早期胚胎发育中所起的重要作用,旨在为提高胚胎质量寻找一条新途径。方法采用脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(TUNEL)检测、天冬氨酸特异性半胱氨酸蛋白酶(Caspase)原位荧光检测和Bcl-2免疫荧光检测等凋亡检测技术,以及增殖细胞核抗原(PCNA)免疫荧光检测细胞增殖程度的技术,分别对小鼠早期形态正常的胚胎、早期发育阻滞的胚胎和有碎片的胚胎进行细胞凋亡程度和增殖程度的检测,比较其凋亡和增殖程度的差异。结果小鼠早期发育阻滞的胚胎细胞和胚胎中的碎片TUNEL阳性,Caspase活性增强,Bcl-2、PCNA表达水平降低。结论细胞凋亡与胚胎发育阻滞及胚胎中的碎片形成密切相关。Objective To investigate the relationship between cell apoptosis and the quality of early mouse embryos,understand the significance of apoptosis-regulatory genes in early embryonic development,and explore a new approach to improving the embryo quality.Methods The levels of cell apoptosis and proliferation in early mouse embryos in different developmental status（morphologically normal embryos,arrested embryos and fragmented embryos） were analyzed with terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling（TUNEL）,caspase in situ fluorescence and Bcl-2 immunofluorescence,and immunofluorescent detection of proliferating cell nuclear antigen（PCNA）.Results The cells in arrested embryos and embryonic fragments showed positive results in TUNEL assay with enhanced caspase activity and lowered expressions of Bcl-2 and PCNA.Conclusion Cell apoptosis in early mouse embryos may be closely related to embryonic arrest and fragmentation.

关 键 词：鼠胚 凋亡 天冬半胱氨酸蛋白酶 BCL-2 增殖细胞核抗原 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]