流式细胞术鉴定特异性识别曲霉孢子单克隆抗体  

Characterization of specific monoclonal antibodies to Aspergillus conidia by flow cytometry

作　　者：余楠[1] 袁小澎[1] 晋晶[1] 郝卫[1] 王艳芳[1] 车小燕[1] 

机构地区：[1]南方医科大学珠江医院医学检验中心,广东广州510282

出　　处：《南方医科大学学报》2011年第3期487-489,共3页Journal of Southern Medical University

基　　金：国家重大科技专项(2009ZX10004-213)~~

摘　　要：目的筛选可以与悬浮状态下曲霉孢子结合的特异性单克隆抗体。方法采用流式细胞术检测与曲霉活孢子结合的单抗。结果 2株单抗MA3和Con2可特异性结合悬浮状态下的烟曲霉孢子,其中单抗MA3可结合烟曲霉、黄曲霉、黑曲霉、土曲霉等常见致病曲霉孢子。结论成功筛选到2株特异性结合曲霉孢子的单抗。Objective To characterize the specific monoclonal antibodies to Aspergillus conidia.Methods Flow cytometry was used to examine the reactivity of the specific monoclonal antibodies to Aspergillus conidia.Results Both the monoclonal antibodies MA3 and Con2 showed specific reactivity to Aspergillus conidia suspensions.MA3 was capable of binding to the conidia of A.fumigatus,A.flavus,A.niger and A.terreus,while Con2 was reactive only to the conidia of A.fumigatus.Conclusion Two specific monoclonal antibodies（MA3 and Con2） to Aspergillus conidia have been obtained.

关 键 词：流式细胞术 曲霉单抗 鉴定 

分 类 号：R392[医药卫生—免疫学]