富血小板血浆对体外培养人脂肪来源干细胞增殖及成脂分化的作用  

Effect of platelet-rich plasma on the proliferation and adipogenic differentiation of human adipose-derived stem cells in vitro

作　　者：张云松[1,2] 何井华[3] 肖冠英[1,4] 黎庆梅[1,4] 

机构地区：[1]暨南大学第四附属医院 [2]广州市红十字会医院整形科,广东广州510220 [3]南方医科大学珠江医院全科医学科,广东广州510282 [4]广州市红十字会医院医学实验中心,广东广州510220

出　　处：《南方医科大学学报》2011年第3期525-528,共4页Journal of Southern Medical University

基　　金：广东省自然科学基金(8451022001001839);广东省医学科学技术研究基金(A2009525);广州市医药卫生科技项目(2008-YB-040)

摘　　要：目的观察富血小板血浆对体外培养的人脂肪来源干细胞增殖与成脂能力的影响。方法用酶消化法获取人脂肪来源干细胞,二次离心法制备自体富血小板血浆,将细胞分为实验组与对照组,实验组以含5%、10%、20%自体富血小板血浆的条件培养液干预,对照组不进行富血小板血浆干预。观察细胞形态学,以XTT比色法测定细胞增殖状况,油红O染色检测细胞成脂活性。结果富血小板血浆实验组较对照组细胞形态饱满、密度增大;XTT比色法显示实验组细胞增殖明显(P<0.01);实验组油红O染色阳性率较对照组增高明显(P<0.01)。结论富血小板血浆对体外培养的人脂肪来源干细胞增殖与成脂分化活性具有显著的促进作用。Objective To observe the effect of platelet-rich plasma（PRP） on the proliferation and adipogenic differentiation of human adipose-derived stem cells in vitro.Methods Human adipose-derived stem cells were obtained by enzymatic digestion and PRP was prepared by dual centrifugal method.The ADSCS were interfused with 5%,10%,and 20% PRP in conditioned culture media,using the untreated cells as the control group.The morphology of the cells were observed and their proliferative ability was detected using XTT colorimetric assay.The adipogenic differentiation ability of the cells was evaluated using oil Red O staining.Results The ADSCS treated with PRP showed better morphology with higher density than the control cells.XTT colorimetric assay demonstrated obviously stronger proliferative activity of PRP-treated cells than the control group（P0.01）.Interfusion with PRP caused a significant increase in adipogenic differentiation of the cells as compared to the control cells（P0.01）.Conculusion PRP treatment produces obvious effects on the proliferation and adipogenic differentiation of human adipose-derived stem cells in vitro.

关 键 词：富血小板血浆 脂肪来源干细胞 细胞增殖 成脂分化 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]