肿瘤坏死因子-α激活大鼠心肌细胞核转录因子-κB活性  

TNF-α activates the nuclear factor-κB activity in rat H9C2 cells

作　　者：高霞[1,2] 马依彤[1] 杨毅宁[1] 向阳[1] 陈邦党[1] 刘芬[1] 杜雷[1] 

机构地区：[1]新疆医科大学第一附属医院心脏中心,新疆乌鲁木齐830054 [2]新疆生产建设兵团医院,新疆乌鲁木齐830000

出　　处：《基础医学与临床》2011年第4期351-354,共4页Basic and Clinical Medicine

基　　金：国家自然科学基金(30760263);新疆自治区卫生厅青年科技人才专项基金(2007Y23);新疆维吾尔自治区重点实验室(XJDX0903)

摘　　要：目的探讨肿瘤坏死因子-α(TNF-α)对大鼠心肌细胞(H9C2)核转录因子-κB(NF-κB)活性的影响。方法体外培养大鼠心肌细胞(H9C2),以TNF-α刺激,凝胶迁移滞后实验(EMSA)检测NF-κB活性,Westen blot及Alamarblue法分别检测P65蛋白与细胞增殖。结果 TNF-α刺激后NF-κB活性明显增高,且TNF-α对细胞生长有抑制作用。结论 TNF-α对大鼠心肌细胞(H9C2)的调节作用,部分是通过激活NF-κB信号通路实现的。Objective To evaluate the effect of tumor necrosis factor α（TNF-α） on nuclear factor-κB（NF-κB） activity in cultured rat H9C2 cells.Methods H9C2 cells were cultured and treated with TNF-α,The quantitative analysis of the protein content and the DNA binding activity of NF-κB were examined by Western blot method and electrophoretic mobility shift assay（EMSA）.Alamar blue assay was used to assess the proliferation of the treated cells.Results TNF-α could stimulate NF-κB activation pathway in H9C2 cells,Alamar blue assay revealed significant difference in absorbance between the treated cells and the control cells.ConclusionSome regulating effect of TNF-α on rats H9C2 cells might be implemented through NF-κB activation pathway.

关 键 词：核转录因子-ΚB 肿瘤坏死因子 大鼠心肌细胞 

分 类 号：R346[医药卫生—基础医学]