促红细胞生成素及其受体在大鼠创伤性颅脑损伤模型中的表达及意义  

Expression and significance of erythropoietin and its receptors in rats with traumatic brain injury

作　　者：贾强[1] 只达石[2] 黄慧玲[2] 蔡英[2] 武俏丽[2] 张学斌[2] 常小丽[2] 

机构地区：[1]天津医科大学第二医院神经外科,300211 [2]天津市环湖医院神经外科

出　　处：《中华创伤杂志》2011年第3期206-209,共4页Chinese Journal of Trauma

基　　金：天津市科技发展计划资助项目（05YFGDSF02500）

摘　　要：目的研究创伤性颅脑损伤（TBI）大鼠损伤周围脑组织促红细胞生成素（erythropoietin，EPO）及其受体（erythropoietin receptor，EPOR）的表达。方法78只sD大鼠按随机数字表法分为对照组（6只）、假手术组（36只）、液压冲击脑创伤模型组（36只）。根据处死时间，分为6，24h和3，5，7，14d6个时相点，每个时相点假手术组和脑损伤组各处死6只大鼠，取损伤周围脑组织。利用实时荧光定量PCR和Western blot方法检测EPO、EPOR的mRNA表达和蛋白表达。结果EPO在伤后24h内即升高，到第3天达到高峰，并可维持2d左右，至伤后第7天开始下降，于伤后14d基本恢复至伤前水平；而EPOR于伤后24h到达高峰，至伤后14d表达量仍可维持较高的水平。结论TBI后24h内源性EPO及其受体的表达即开始增加，但二者的表达具有不一致性，且EPO相对受体表达具有短暂性。Objective To study the expressions of erythropoietin （EPO） and its receptors （EPOR） in the injured brain tissue of the rats with traumatic brain injury （TBI）. Methods A total of 78 SD rats were randomly divided into three groups including control group （six rats） , sham group （36 rats） and fluid percussion injury group （36 rats）. The rats were sacrificed at 6,24 hours, 3,5,7 and 14 days after TBI in the sham group and the fluid percussion injury group （ six rats at each time point ）. Then, the injured brain tissues were removed for observation of the mRNA and protein expressions of EPO and EPOR by means of real-time PCR and Western blot. Results The expression of EPO was increased at 24 hours and reached the peak at day 3 after TBI. The high expression level of EPO could maintain for two days or so, began to decrease at day 7 and recovered to normal at day 14 after TBI. While the expression of EPOR reached the peak at 24 hours after TB[ artd maintained high level at day 14. Conclusions The expressions of EP0 and EPOR show increase within 24 hours after TBI. In fact, the expressions of both factors are not in consistency, with more transient expression of EPO.

关 键 词：脑损伤 促红细胞生成素 受体 

分 类 号：R651[医药卫生—外科学] R-332[医药卫生—临床医学]