舒芬太尼后处理对在体大鼠心肌缺血再灌注损伤的影响  

Effects of Sufentanil Postconditioning on Myocardial Ischemia-Reperfusion Injury in Rats in Vivo

作　　者：吴云[1,2] 顾尔伟[1] 方卫平[1] 朱燕[1] 张雷[1] 刘训芹[2] 

机构地区：[1]安徽医科大学第一附属医院麻醉科,合肥230022 [2]安徽医科大学第二附属医院麻醉科,合肥230601

出　　处：《麻醉与监护论坛》2010年第6期440-442,共3页Forum of Anesthesia and Monitoring

摘　　要：目的：探讨舒芬太尼后处理对在体大鼠心肌映血再灌注损伤的影响。方法：健康雄性S—D大鼠48只．体重230～330g．结扎冠状动脉左前降支制备心肌缺血再灌注损伤模型。随机分为B组（n=6）：假手术组（sham组）：只穿线．不结扎；缺血再灌注组（CON组）：缺血30min．再灌注120min，再灌注前5min单次静脉注射生理盐水1mL；缺血后处理组（IpostC组）：缺血30min末行缺血10s．再灌注10s，重复3次后再灌注120min；舒芬太尼后处理组（0．1SpOStC组～10SpostC组）：再灌注前5min分别单次静脉注射舒芬太尼0．1．0．3，1、3．10ug／kg．5min后再灌注120min。于结扎线缝好后平衡30rnfn（T0）、缺血30min末（TI），后处理末（T2）．再灌注120min末（T3）记录MAP-HR．并计算血压心率乘积（RPP）。计算心肌梗死面积（1S）与缺血危险区（AAR）比值（IS／AAR）。选取最佳剂量舒芬太尼后处理组．sham组．CON组．IpostC组于T3时取颈动脉血2ml。采用硫代巴比妥酸（TBA）法测定丙二醛（MDA）浓度．黄嘌呤氧化酶法测定超氧化物歧化酶（SOD）活性。结果：与CON组比较．IpostC组、0．3．1．3、10SpostC组IS／AAR降低（P〈0．05）。其中舒芬太尼后处理组中1SpostC组降低最为明显；与IpostC组比较．0．1SpostC组IS／AAR增加（P〈0．05）。舒芬太尼剂量-效应关系si9mOidal方程为：Y=0．3749＋0．4872／。（1＋10^1.502-x）．ED50为0．03174ug／kg。与sham组比较，其余三组血清MDA浓度升高．SOD活性降低（P＜0．05）；与CO嘲比较．IpostC．、SpostC组MOA降低．SOO话性升高（P〈0．05）。结论：舒芬太尼可模拟缺血后处理减轻在体大鼠心肌缺血再灌注损伤．并且呈剂量依赖性。Objective To investigate effects of sufentanil postconditioning（SpostC） on myocardial ischemia-reperfusion（l/R） injury in rats. Methods Forty-eight male S-D rats weighing 230-330g were anesthetized with intraperitoneal 5% pentobarbital 40 mg/kg,tracheostomized and mechanically ventilated.Right carotid artery and jugular vein were cannulated for BP monitoring and drug administration.ECG,MAP, and HR were monitored continuously. A 6-0 proline ligature was placed under the left anterior descending （LAD） coronary artery and threaded through a small plastic tube to form a snare for reversible LAD artery occlusion.The body temperature was maintained constantly between 37-38℃ by a heating pad. The animals were then randomized into 8 groups： I Rats received no occlusion or reperfusion（group sham）; Ⅱ Rats were subjected to 30 min ischemia and 120 min reperfusion,for a negative control group, normal saline 1 ml was infused at the 25 min of ischemia （group CON）;Ⅲ Immediately at the onset of reperfusion,rats underwent 3 intermittent cycles of 10s reperfusion alternating with 10s ischemia （group IpostC）;Ⅳ-Ⅷ Rats received at random an intravenous infusion of sufentanil diluted with normal saline 1 ml of either dosage（0.1,0.3,1,3,10 pg/kg） at the 25 min of ischemia（group 0.1SpostC-group 10SpostC）.MAP, HR and RPP were recorded after a 30 min stabilization（T0）,at the end of ischemia（T1）,at the end of postconditioning（T2） and at the end of reperfusion（T3）.The infact size （IS） and the area at risk （AAR） were measured by TTC staining. Serum MDA concentration and SOD activity were determined in group sham,group CON,group IpostC and group optimal sufentanil postconditioning. Results： IS/AAR was significantly decreased in the treatment groups except group 0.1SpostC compared with group CON and group 1SpostC has the optimal protective effect in the sufentanil postconditioning groups（P〈0.05）.The sigmoidal equation of the dose-effect curve was Y=0.3749＋0.4872/ �

关 键 词：舒芬太尼 缺血后处理 缺血/再灌注损伤 心肌 

分 类 号：R542.2[医药卫生—心血管疾病]