静脉输注高氧液对急性一氧化碳中毒大鼠生化指标的影响  

The Effects of Hyperoxygen Solution Injected in Vein on Biochemical Indexes of Rats with Acute Carbon Monoxide Poisoning

作　　者：徐浩 孟祥忠 高巍 宋博 徐礼鲜 张惠 

机构地区：[1]第四军医大学口腔医院麻醉科,710032

出　　处：《麻醉与监护论坛》2010年第6期447-449,共3页Forum of Anesthesia and Monitoring

摘　　要：目的：本研究旨在探讨静脉输注高氧液（HOS）对急性一氧化碳（CO）中毒大鼠生化指标的影响。方法将30只SD大鼠随机分为5组，每组6只。正常组cN组）、中毒组（c组）和中毒治疗组（H组），审毒治疗组再根据输注HOS剂量分为．在中毒后1h输注HOS 20ml／kg（H2O组）、25ml／kg（H25组）和30ml／kg（H30组）。C组与各治疗组大鼠经腹腔注入CO120mlAg建立中毒模型．N组给予同等剂量的空气。各组大鼠分别在输注不同剂量的液体后即刻抽取动脉血0．5ml进行血气指标检测．染毒后1d抽取静脉血3ml进行脑、心．肝、肾敏感生化指标的测定。结果：CO120ml／kg腹腔注射1．5h能引起严重的低氧血症PaO2由96.62±2．4mmHg降低到43．04±4．13mmHg。中毒后1d能够引起神经元特异性烯纯化酶（NSE），S-100D蛋白．肌酸激酶（CK）．肌酸激酶同工酶（CK-MB），乳酸脱氢酶（LDH）、α-羟丁酸脱氢酶（α-HBDH）．总胆红素（TBIL）、谷草转氨酶（AST）、谷丙转氨酶（ALT）．谷氨酰转移酶（GGT）、尿素氮（BUN）和肌酐（cr）的明显升高．CO中毒组与正常对照组比较有显著差异（P〈0．01）。静脉输注不同剂量的HOS后。血浆溶解氧的含量显著升高，PaO2由43．04±4．13mmHg上升到81．65±3．85mmHg。各种生化指标也均有不同程度的降低。虽然明显高于正常对照组．但与CO中毒组比较各种指标的升高均有所降低（P〈0．05或P〈0．01），其中H30组降低最明显．具有显著的剂量依赖关系。结论：经静脉输注HOS能降低急性CO中毒大鼠血浆各种生化指标的含量。对急性CO中毒引起的重要脏器损害具有很好的保护作用，并具有剂量依赖关系。Objective： To investigate the effects of hyperoxygen solution （HO） injected in vein on biochemical indexes of rats with acute carbon monoxide poisoning. Methods 30 SD rats were randomly divided into 5 groups, 6 in each group. Normal group （N group）, carbon monoxide poisoning group （C group） and different dose of HO treatment groups at lh after CO poisoning, 20ml/kg （H20 group）, 25ml / kg （H25 group） and 30ml/kg （H30 group）. CO poisoning model were established by intraperitoneal injection CO with 120ml/kg. C group and the treatment group were such poisoning rats, while N group were given the same dose of air. At the end of treatments, all the rats were collected 0.5ml of arterial blood for blood gas measurement and 3 ml of venous blood for the determination of sensitive biochemical indexes in brain, heart, liver, and kidney. Results CO 120ml/kg with intraperitoneal injection can cause severe hypoxemia after 1.5 hours, PaO2 decreased from the 96.62 ± 2.4 mmHg to 43.04 ± 4.13mmHg. After CO poisoning for ld, C group had significantly increase in Neuron-specific enolase （NSE）, troponin （cTnT）, creatine kinase （CK）, creatine kinase ME] （CK-MB）, lactate dehydrogenase （LDH）, a-hydroxybutyrate dehydrogenase （a-HBDH）, total bilirubin （TBIL）, aspartate aminotransferase （AST）, alanine aminotransferase （ALI）, glutamyl transferase （GGT）, blood urea nitrogen （BUN） and creatinine （Cr） （P 〈0.01）. When intravenous infused with different doses of HOS, Dissolved oxygen in plasma was significantly increased, PaO2 increased from the 43.04 ± 4.13 mmHg to 81.65± 3.85mmHg.AII the enzymes were significantly lower than these in C group, although still were significantly higher than these in N group（P〈0.05 or P〈0.01）. Moreover, all the enzymes were significantly lowest in group H30 with a significantly dose dependent relationship. Conclusion Intravenous infusion with hyperoxygen solution can reduce the biochemical indexes changes caused by acute C

关 键 词：一氧化碳中毒 高氧液 生化指标 

分 类 号：R595.1[医药卫生—内科学]