郏县红牛超数排卵研究  

Study on Superovulation in Jiaxian Red Cattle

作　　者：王新庄[1] 和小娥[1] 张静芳[2] 石奎林 何俊丹[1] 秦佳晨[1] 任卫青[1] 曹桂彬[1] 

机构地区：[1]河南农业大学牧医工程学院,郑州450002 [2]西北农林科技大学动物医学院,陕西杨凌712100 [3]平顶山市畜牧局,河南平顶山467000

出　　处：《西北农业学报》2010年第12期1-4,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：河南省杰出人才创新基金(0421002100);"863"高新技术项目(2008AA101010);现代农业产业技术体系专项资金;农财发[2008]58号

摘　　要：应用促卵泡素(FSH)连续4 d递减法肌肉注射郏县红牛进行超数排卵处理,重点探讨药物剂量、药物产地、季节以及重复超排等因素对郏县红牛超排的影响。结果表明,用加拿大FSH在300 mg剂量时的头均获胚总数和头均可用胚数(8.86和5.98枚)显著高于280 mg剂量组(6.32和3.05枚)和320 mg剂量组(6.56和4.12枚)(P<0.05);用宁波产FSH在320 I U剂量条件下的头均获胚总数和头均可用胚数(8.12和5.46枚)显著高于280 I U组(5.55和2.76枚)和300 I U剂量组(6.32和3.77枚)(P<0.05)。综合3个剂量的处理结果,进口FSH和国产FSH对郏县红牛的超排处理效果无显著差异,头均黄体数分别为13.89个和13.41个,头均获胚总数分别为6.94和6.74枚,头均可用胚数为4.42和3.95枚;在夏秋季(5-10月)超数排卵郏县红牛23头次,冬春季(11-次年4月)超数排卵郏县红牛21头次,发现超排季节对头均黄体数无显著影响(P>0.05),但冬春季头均获胚总数(7.23枚)和头均可用胚数(6.51枚)显著高于夏秋季(4.57和2.90枚)(P<0.05);对郏县红牛供体牛间隔70 d重复超排处理后发现,1次、2次和3次重复超排头均黄体数分别为14.30、13.12和12.23个,头均获胚总数分别为7.31、6.52和6.45枚,头均可用胚数分别为4.56、4.15和3.96枚,重复超排对上述3个指标均无显著影响(P>0.05)。To study the effect of drug,dosage,season and repeat superovulation on superovulation of Jiaxian red cattle.FSH was applied to superovulate by intramuscular injection on Jiaxian red cattles for continual 4 days decrement.The result shows that the average embryoes and available embryoes of Canada FSH at the dosage 300mg per head were,respectively,8.86 and5.98,which were significantly higher than that of dosage 280 and 320 mg per head（6.32,3.05 and 6.56,4.12,respectively）（P0.05）.The average embryoes and available embryoes of domestic（Ningbo） FSH at the dosage 320 mg per head were,respectively,8.12 and 5.46,which were significantly higher than that of dosage 280 and 300mg per head（5.55,2.76 and 6.32,3.77,respectively）（P0.05）.After and autumn（May to October） as well as winter and spring（November to April）,twenty-three and twenty-one jiaxian red cattle were superovulated,respectively.The results showed that season had no significant effect on average CL（P0.05）.Higher average embryo and available embryo were obtained in winter and spring（7.23 and 6.51） than in summer and autumn（4.57 and 2.90）（P0.05）.At interval of 70 days,the average CL of once,twice and thrice superovulating treatment were 14.30,13.12 and 12.23,respectively,and the average embryo and available embryo were 7.31,6.52 and 6.45 as well as 4.56,4.15 and 3.96,respectively.It can be indicated that the time of superovulating treatment had no effect on the above three index（P0.05）.

关 键 词：超数排卵 黄体 胚胎 郏县红牛 

分 类 号：S823.36[农业科学—畜牧学]