转基因沉默HMW-GS基因的遗传规律及其在育种中的应用  

Genetic behavior and application of transgenic silenced HMW-GS genes in wheat

作　　者：武茹[1,2] 别同德[2] 高德荣[2] 张晓[2] 赵芸[2,3] 程顺和[2] 

机构地区：[1]扬州大学江苏省作物遗传生理重点实验室/教育部植物功能基因组学重点实验室,江苏扬州225009 [2]江苏里下河地区农业科学研究所,江苏扬州225007 [3]扬州大学生物科学与技术学院,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2010年第4期31-35,共5页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家重点基础研究发展计划项目(973-2009CB118300);南方麦区弱筋小麦转基因品质改良项目(2008ZX08002-004);小麦现代农业产业技术体系资助项目(NYCYTX-03)

摘　　要：以转基因小麦Glu-1-RNAi为供体亲本、弱筋品种扬麦18和扬麦13为受体亲本进行常规杂交与回交,采用半籽粒SDS-PAGE技术检测并分析亲本、Fl、F2、F3、BClF1、BC1F2、BC2Fl世代高分子量谷蛋白亚基(High molecularweight glutenin subunit,HMW-GS)的组成,研究由RNA干扰技术诱导的HMW-GS沉默效应在非转基因小麦品种中的遗传规律。结果表明:转基因小麦HMW-GS的沉默效应表现为显性遗传,在自交及回交后代中HMW-GS的沉默效应能够稳定遗传。HMW-GS的沉默效应在弱筋小麦培育工作中具有应用价值。High-molecular-weight glutenin subunits（HMW-GS） play a central role in wheat kernel quality.To investigate the genetic behavior of silenced HMW-GS genes,transgenic wheat line named ＂Glu-1-RNAi＂ with silent HMW-GS genes was firstly crossed with two soft wheat varieties Yangmai 18 and Yangmai 13 which were employed as re-current parents in the following backcrosses.HMW-GS distribution in parent lines and their progenies,including F1,F2,F3,BC1F1,BC1F2 and BC2F1 were analyzed by the SDS-PAGE technique.Results showed that the silent effect of HMW-GS genes was dominant and could stably inherit in progenies.Results presented in this study also indicated that the silent effect of HMW-GS genes could be widely used in weak glutein wheat breeding programmes.

关 键 词：小麦 RNA干扰 HMW-GS基因 转基因沉默 遗传 

分 类 号：S512.032[农业科学—作物学]