樱桃番茄闭药雄性不育基因的RAPD标记  

The RAPD Markers of the Anther-indehiscent Male Sterile Gene in Cherry Tomato

作　　者：李海涛[1] 王晓峰[2] 吕书文[1] 杨国栋[1] 吴媛媛[3] 

机构地区：[1]辽宁省农业科学院,沈阳110161 [2]沈阳农业大学园艺学院,沈阳110866 [3]辽宁省农业科学院经济作物研究所,辽宁辽阳111000

出　　处：《沈阳农业大学学报》2010年第5期610-613,共4页Journal of Shenyang Agricultural University

基　　金：科技部国家科技支撑计划项目(2006BAD01A7-3-05)

摘　　要：为了获得与樱桃番茄闭药不育基因紧密连锁的RAPD(random amplified polymorphic DNA,即随机扩增多态性DNA)标记,以大果型闭药雄性不育系(94-205)为父本,以樱桃番茄自交系(02-281)为母本,构建了132个F2分离群体。采用RAPD分子标记技术,通过BSA法筛选230条RAPD引物,获得了1个可用于樱桃番茄闭药不育的标记CCPA931-031225(序列为TGCGTGCTTG),RAPD标记CCPA931-031225与闭药不育基因遗传图距为5.6cM,为紧密连锁。这个标记的获得,可用于标记辅助选育,加速番茄闭药雄性不育的转育和制种应用。With the objective of getting the RAPD（random amplified polymorphic DNA） marker of the gene tightly linked the Anther-indehiscent Male Sterile gene,132 individuals of F2 segregated population were derived from a cross between a cultivation of cherry tomato line（02-281） as a male line and an Anther-indehiscent Male Sterile Mutant line（94-205） as a female line.The method of RAPD marker was used,and with the method BSA to search for RAPD markers linked to gene,230 RAPD primer combinations were screened.The result was one RAPD marker could be used to mark the Anther-indehiscent Male Sterile gene CCPA931-03（the sequence of the primer： TGCGTGCTTG）,the RAPD marker CCPA931-03 was 5.6cM from the Anther-indehiscent Male Sterile gene and they were tightly linked.The RAPD markers obtained could be directly used for marker-assistant selection,speed up the Anther-indehiscent Male Sterile transfer and the use for tomato hybrid F1 production.

关 键 词：樱桃番茄 闭药不育 RAPD标记 

分 类 号：S641.2[农业科学—蔬菜学]