纳米ZnO对鲫鱼肝脏的毒性  

Toxicity of Nano-ZnO on Liver of Goldfish(Carassius auratus)

作　　者：刘慧[1] 朱方伟[1,2] 尹颖[2] 郭红岩[2] 孙媛媛[2] 王晓蓉[2] 

机构地区：[1]河海大学水利水电学院南方地区高效灌排与农业水土环境教育部重点实验室,南京210098 [2]南京大学环境学院污染控制与资源化研究国家重点实验室,南京210093

出　　处：《生态毒理学报》2010年第5期698-703,共6页Asian Journal of Ecotoxicology

基　　金：国家自然科学基金青年项目(No.20907020)

摘　　要：鲫鱼(Carassius auratus)腹腔注射不同浓度纳米ZnO(5mg·kg-1、12.5mg·kg-1、25mg·kg-1、50mg·kg-1和125mg·kg-1,以鲫鱼体重计)14d后,研究了鲫鱼肝脏中的自由基(ROS)强度变化、氧化应激反应及其毒性机制.结果表明:纳米ZnO显著诱导鲫鱼肝脏自由基产生;自由基信号强度和脂质过氧化物(MDA)随纳米ZnO浓度的升高呈先升高后降低趋势;而还原型谷胱甘肽(GSH)含量和GSH/GSSG随纳米ZnO浓度的升高呈先降低后升高趋势;纳米ZnO的毒性主要表现在引起鲫鱼肝脏氧化损伤,其毒性机制为诱导鲫鱼肝脏产生活性氧自由基.After 14 days of Nano-ZnO intraperitoneal injection which concentration is 5mg·kg-1、12.5mg·kg-1、25mg·kg-1、50mg·kg-1 and 125mg·kg-1,the free radicals generation,oxidative stress and toxic mechanism on Carassius auratus liver have been studied.The results show that Nano-ZnO significantly promoted the generation of free radicals in C.auratus liver.Signal intensity of free radicals and the content of lipid peroxides（MDA）firstly dropped and then raised with the increase of the concentrations of Nano-ZnO.While the reducibilityglutathione（GSH）content and GSH/GSSG firstly raised and then dropped with the increase of the concentrations of Nano-ZnO.Nano-ZnO caused the oxidative damage for Carassius auratus liver.The main toxic mechanism is that Nano-ZnO made the fish liver producing free radicals.

关 键 词：纳米ZNO 自由基 脂质过氧化物 超氧化物歧化酶 谷胱甘肽 

分 类 号：X503.225[环境科学与工程—环境工程]