日本血吸虫抗体检测试剂盒(间接血凝法)应用效果评价  

Effective evaluation of IHA kit in the diagnosis of schistosomiasis

作　　者：王恩木[1] 章乐生[1] 张世清[1] 郝晓君[1] 呼明闯[1] 

机构地区：[1]安徽省血吸虫病防治研究所,芜湖市241000

出　　处：《热带病与寄生虫学》2009年第4期203-205,共3页Journal of Tropical Diseases and Parasitology

基　　金：安徽省高新技术产业化项目(2005-33)资助

摘　　要：目的评价日本血吸虫抗体检测试剂盒(间接血凝法)在实际应用中的效果。方法采用单盲法,分别检测血吸虫、肺吸虫、肝吸虫、旋毛虫、结核、不明原因发热等病人血清和健康人群血清。结果检测100份慢性血吸虫病人血清的敏感性为97.0%;检测50份健康人血清的特异性为96.0%;检测20份华支睾吸虫病人血清的交叉反应率为10.0%;检测10份肺吸虫病人血清的交叉反应率为50.0%;检测10份旋毛虫病人血清的交叉反应率为30.0%;检测乙肝、鞭虫、蛔虫、钩虫病人血清均为阴性;检测18份结核病人血清的交叉反应率为16.67%;检测88份不明原因发热病人的交叉反应率为11.36%;检测473份非疫区人群血清的交叉反应率为3.38%;检测1136份传播阻断地区人群血清的交叉反应率为2.29%。结论日本血吸虫抗体检测试剂盒(间接血凝法)在血吸虫病检测中具有较高的灵敏性和特异性,可用于血吸虫病现场筛查。Objective To evaluate the effect of IHA kit in the diagnosis of schistosomiasis. Methods The sera from patients with schistosomiasis, judge paragonimiasis, hepatic distomiasis, trichinellosis, tuberculosis, fever of undetermined origin and healthy people were examined respectively by using IHA kit according to the principle of single-blind. Results A total of 100 sera from patients with chronic schistosomiasis were examined, and the sensitivity was 97.0%. A total of 50 sera from healthy people were examined, and the specificity was 96. 0%. A total of ：20 sera from patients with clonorchis sinensis were examined, and the cross-reaction rate was 10. 0%. A total of 10 sera from patients with paragonimiasis were examined, and the cross-reaction rate was 50. 0%. A total of 10 sera from patients with trichinellosis were examined, and the cross-reaction rate was 30. 0%. There were no positives for the patients with HBV, whipworm, roundworm and hookworm patients. The cross-reaction rate was 11.36% for the patients with fever of undetermined origin, and the cross-reaction rate was 3.38% for the people from non-endemic area of schistosomiasi, and. the cross-reaction rate was 2. 29% for the people from schistosomiasis transmission interrupted area. Conclusion IHA kit has high sensitivity and specificity in the diagnosis of schistosomiasis, and it can be used in the field for the disease screening.

关 键 词：日本血吸虫 IHA 特异性 敏感性 

分 类 号：R532.21[医药卫生—内科学]