高效液相色谱法测定金艾康中汉防已甲素的含量  

Determination of tetrandrine in Jinake by HPLC

作　　者：于伟健[1] 蔡永葆[1] 蒋礼年 

机构地区：[1]上海市徐汇区中心医院,上海200031 [2]浙江康恩贝集团有限公司,杭州310003

出　　处：《中国现代应用药学》1999年第4期50-52,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的 :建立一种准确度和灵敏度高 ,重现性好的汉防已甲素高效液相色谱测定法。方法 :采用ODSC18( 4.6mm× 1 50mm)为分析柱 ,流动相 :十二烷基磺酸钠 乙腈 0 .1mol/L磷酸盐缓冲液 (pH2 ) ( 0 .1 5∶50∶50 ) ,检测波长24 0nm。结果 :含量测定汉防已甲素在 0 .0 6～ 0 .3 0 μg线性关系良好 ,回收率 99.9% ,RSD为 1 .66%。 结论 :建立的含量测定方法简便、准确、灵敏。OBJECTIVE:To establish a accurate sensitive and reproducible method for the determination of tetrandrine in Jinake.METHODS:The column was ODS C 18 (4.6mm×150mm);mobile phase was sodium dodecyl sulfate aceetonitrile 0.01mol/L phosphoric acid buffer(pH2)(0.15∶50∶50);detection wave length was 240nm.RESULTS:The recovery 99.9% and linear range were 0.06～0.30μg,RSD 1.66%.CONCLUSION:The method is simple,accurate and sensitive for the determination of tetrandrine in Jinake.

关 键 词：金艾康 汉防己甲素 含量测定 高效液相色谱 

分 类 号：R971.1[医药卫生—药品] R927.2[医药卫生—药学]