两型豆种子发育的解剖学研究  

Anatomic Study on the Seed Development of Amphicarpaea edgeworthii Benth.

作　　者：张立峰[1] 佘跃辉[1] 

机构地区：[1]四川农业大学农学院,四川雅安625014

出　　处：《种子》2011年第3期53-56,共4页Seed

基　　金：四川省"十一五"农作物育种攻关项目;四川省教育厅育种专项(08ZZ001)

摘　　要：两型豆为豆科野生物种,对大豆育种和植物发育生物学研究具有重要价值。试验采用石蜡切片法进行了两型豆种子发育的解剖学研究,结果表明:(1)胚珠在花后12 h完成受精;(2)花后2 d合子进行第1次分裂,第1次分裂为横分裂,花后4 d形成原胚;(3)花后24 d,原胚经过球形胚期、心形胚期、鱼雷胚期,发育为成熟胚;(4)胚乳发育为核型,成熟种子为无胚乳种子;(5)外珠被发育为种皮,种皮细胞呈栅栏状且外被角质层,是造成三籽两型豆地上种子硬实的重要原因之一。Amphicarpaea edgeworthii Benth.is a wild species of the legume,with important value on the soybean breeding and researchs of plant developmental biology.The seed development of Amphicarpaea edgeworthii Benth.were observed using paraffin sectioning method by microscope.The ovule was fertilized and formed the zygote after flowering about 12 hours.The zygote was transfersely divided into a terminal cell and a basal cell in 2 days after flowering.The proembryo continued cell division in very directions forming globular embryo and then heart-shaped embryo,in which the cotyledons primordium began to occur.In 24 days after flowering,the embryo differentiated into matured embryo through Tropedo-shaped embryo（in 16 days after flowering）.The endosperm cell began to form when the globular proembryo was formed,and the development of the endosperm was nuclear type.The mature seed of Amphicarpaea edgeworthii Benth is the seed without endosperm.The outer integument developed into the seed coat,and the seed coat cells were the fence cells with corneum,that might be one of the important reasons of hard seeds for Amphicarpaea edgeworthii Benth.

关 键 词：三籽两型豆 解剖 发育 种子 

分 类 号：S58[农业科学—作物学]