兴奋或抑制细胞内PKC或cAMP对不同脉冲频率GnRH刺激下GTH表达水平的影响  

Influence of excitement or inhibition-induced PKC or cAMP in cells on the expression of GTH after stimulation with GnRH at different impulse frequencies

作　　者：万忠海[1] 王新[1] 谭建华[1] 

机构地区：[1]军事医学科学院军事兽医研究所,吉林长春130062

出　　处：《中国兽医杂志》2011年第3期6-8,共3页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金资助项目(30571356)

摘　　要：观察兴奋或抑制细胞内cAMP和PKC后,LH和FSH对不同脉冲频率GnRH刺激的应答情况,分析LH/FSH表达的受体后信号转导机制。结果表明,使用cAMP的兴奋剂或抑制剂使细胞cAMP含量升高或降低时,显著影响了LH的应答,但对FSH的应答影响不显著;使用PKC的兴奋剂或抑制剂使细胞PKC活性升高或降低时,既显著影响了LH的应答,也显著影响了FSH的应答。两者均随着PKC活性的升降而升降。To observe the response features of LH and FSH to the stimulation of GnRH at different impulse frequencies after PKC or cAMP in cells were excited or inhibited.The result of the experiment showed that the use of the activator and the inhibitor of cAMP made the cAMP level rise or fall,the expression of LH is influenced significantly,while the influence on the expression of FSH was not striking;the use of the activator and the inhibitor of PKC makes the PKC level rise or fall,not only the expression of LH but also the expression of FSH is influenced significantly.The levels of both rise with the rise of PKC and falls with the falling of PKC.

关 键 词：兴奋 抑制 脉冲频率 信号转导 

分 类 号：S852.2[农业科学—基础兽医学]