禾本科植物内生真菌研究12:吉林犬草内生真菌的分离和鉴定  

Isolation and identification of a fungal endophyte obtained from Roegneria canina(L.) Nevski grown in Jilin,China

作　　者：张晨炜[1] 纪燕玲[1] 杨守玲[1] 亢燕[1] 王志伟[1] 

机构地区：[1]南京农业大学生命科学学院,江苏南京210095

出　　处：《南京农业大学学报》2011年第2期78-84,共7页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(30670008;30800156)

摘　　要：在我国吉林省长春市3个采样点共采集96株鹅观草属植物样品,经鉴定为犬草(Roegneria canina)。通过检测,样品中内生真菌的含菌率高达67.7%。分离得到5个菌株并对它们进行形态学特征调查,分离菌株呈典型的Neotyphodium属真菌特征。选择菌株Rjl7101和Rjl7104,利用tefA和tubB基因片段进行系统发育分析,结果表明分离菌株与Neotyphodiumsinicum聚为一枝。根据以上结果,确定分离菌株为N.sinicum。这是首次在犬草中发现N.sinicum,也扩大了N.sinicum在我国的分布地区范围,进一步说明我国拥有极为丰富的内生真菌资源且分布广泛。Ninety-six Roegneria samples were collected from 3 sites in Jilin Province.They were identified as R.canina.Fungal hyphae were detected from 67.7% of them,with no obvious difference among each site.Five fungal isolates were isolated from these samples through seedling.Their morphological properties were typical in Neotyphodium spp..Phylogenetic relationships based on sequences of β-tubulin gene（tubB）introns and translation elongation factor 1-α gene（tefA）introns of Rjl7101 and Rjl7104 revealed that they clustered together in a distinct sub-clade in the Neotyphodium sinicum clade.It indicated these isolates should be N.sinicum.R.canina is thus a new plant host of N.sinicum.So N.sinicum was also discovered in northeast of China.Epichlo-grass symbioses are broadly distributed in China.

关 键 词：犬草 NEOTYPHODIUM sinicum 形态学特征 系统发育分析 

分 类 号：Q939.5[生物学—微生物学]