转人乳铁蛋白基因山羊乳对大鼠生长及代谢机能的影响  

Effect of transgenic human lactoferrin goats′ milk on growth and metabolic performance in rats

作　　者：王二先[1] 徐旭俊 苗晋锋[1] 陈建泉 成国祥 杨倩[1] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095 [2]上海转基因研究中心,上海201203

出　　处：《南京农业大学学报》2011年第2期119-123,共5页Journal of Nanjing Agricultural University

基　　金：转基因生物新品种培育重大专项(2008ZX08008-04;2009ZX08011-032B)

摘　　要：选用30只100 g左右清洁级SD大鼠,雌雄各半,随机均分为3组,转基因乳组每天2次灌胃转人乳铁蛋白基因(hLF)波尔山羊乳1 mL(蛋白含量0.93 g.mL-1),普通乳组灌胃未经任何处理的普通波尔山羊乳1 mL(蛋白浓度0.93 g.mL-1),生理盐水组灌胃等量灭菌生理盐水;自由采食、饮水,连续15 d;颈部静脉采血致死,收集血清。结果显示:灌胃转hLF基因山羊乳显著提高了雌、雄大鼠的日增重,与生理盐水组相比差异极显著(P<0.01),与普通乳组相比差异显著(P<0.05),而普通乳组与生理盐水组相比差异不显著;灌胃转基因和普通山羊乳对其他表观及生化指标均无显著影响(P>0.05)。试验结果表明:短期灌胃转hLF基因山羊乳能够促进大鼠的生长,但对大鼠的代谢机能没有显著影响。Fifteen healthy SD female rats and fifteen healthy male rats weighting about 100 g were randomly distributed into three groups.The transgenic milk group was infused with transgenic human lactoferrin gene（hLF）goats′ milk 1 mL（protein concentration at 0.93 g·mL-1）,directly into the gastric two times a day;the normal milk group with normal goats′ milk（protein concentration at 0.93 g·mL-1）,and the physiological saline group with sterile pyrogen-free physiological saline at the same quantity.Three groups were provided a standard rodent chow and an ad libitum supply of water.After 15 days of infusing,all the rats were killed by collecting living body blood in venous of neck.The result indicated that the average daily gain of the female and male rats which were infused with transgenic hLF goats′ milk increased in evidence compared to the physiological saline group（P0.01）and the normal milk group（P〈0.05）.However,there were no difference between the normal milk group and the physiological saline group.And the other measurements and biochemical examination of the transgenic milk group and the normal milk group didn′t change significantly compared to the physiological saline group（P〉0.05）,moreover,no obvious change were observed between the transgenic and normal milk group.Overall,the result of the present study demonstrates that transgenic hLF goats′ milk can enhance rats growth performance but has no effect on its metabolism by infusing administration in short time.

关 键 词：人乳铁蛋白 转基因山羊乳 大鼠 代谢 

分 类 号：S879.1[农业科学—畜牧兽医] S816.48