单胺氧化酶基因A和B的二种多态与高血压的关系  

The Relationship Between Hypertension and the Two Polymorphisms of Monoamine Oxidase A and Monoamine Oxidase B

作　　者：郭威早[1] 唐邦熙[1] 常丙习 王军英[1] 王莉瑛[1] 刘瑞雪[1] 冯雪英[1] 叶大雄[1] 徐南图[1] Bill Wu Theodore Reich 

机构地区：[1]邮电总医院内科,北京市100032 [2]美国华盛顿大学医学院

出　　处：《中国循环杂志》1999年第4期216-218,共3页Chinese Circulation Journal

摘　　要：目的：探讨单胺氧化酶（ＭＡＯ）基因多态性与高血压发病的关系。　　方法：按世界卫生组织标准收集高血压患者１０４例为高血压组，正常对照组１０１名。利用聚合酶链式反应（ＰＣＲ）和凝胶电泳技术对所有个体进行ＭＡＯ基因多态性分析，所检测的基因包括ＭＡＯ－Ａ的一个限制性片段长度多态（ＲＦＬＰ）和ＭＡＯ－Ｂ的一个二核苷酸重复多态（ＤＮＲＰ）。采用统计学方法探讨ＭＡＯ基因多态性与高血压发病的关系。　　结果：ＭＡＯ－Ａ 基因对于内切酶ＥｃｏＲＶ 的２ 个ＲＦＬＰ等位基因在高血压组和正常对照组的分布无显著差异；ＭＡＯ－Ｂ基因所表现的７种ＤＮＲＰ等位基因中，１９５ ｂｐ 和１９９ ｂｐ 片段在高血压组和正常对照组中表现出显著差异（χ２检验，Ｐ＜ ０．０５）。　　结论：ＭＡＯ－Ｂ基因多态可能影响高血压发病，其机制需进一步研究。Objective:To investigate the relationship between monoamine oxidase(MAO)gene polymorphisms and the pathogenesis of hypertension. Methods:One hundrend and four patients fulfill the criteria of hypertension of World Health Organization(WHO)were collected while 101 normal persons were adopted as controls.Polymerase chain reaction(PCR)and gel electrophoresis were used to detect the genotype of MAO gene.The target loci selected in this research include a restriction fragment length polymorphism(RFLP)of MAO A gene and a dinucleotide repeat polymorphism(DNRP)of MAO B gene.The relationship between hypertension and polymorphisms of MAO gene was analyzed by statistical method. Results:The distribution of the two alleles at the polymorphic EcoR V site of MAO A gene did not have statistical difference between patients with hypertension and normal controls while the frequencies of the two DNRP alleles(195 bp and 199 bp)of MAO B gene in patients and controls showed statistical difference( χ 2 test, p <0 05). Conclusion:Some polymorphisms of MAO B gene may have a predisposition to hypertension,the mechanism need to be detected further.

关 键 词：高血压 单胺氧化酶 基因多态性 

分 类 号：R544.1[医药卫生—心血管疾病]