石杉碱甲及其制剂的HPLC测定  

DETERMINATION OF HUPERZINE A AND ITS PREPARATION BY HPLC

作　　者：于伟健[1] 余琛[1] 张慧[1] 王昕[2] 蒋山好[2] 朱大元[2] 

机构地区：[1]上海市徐汇区中心医院,上海200031 [2]中国科学院上海药物研究所,上海200031

出　　处：《中国医药工业杂志》1999年第8期363-365,共3页Chinese Journal of Pharmaceuticals

摘　　要：用Ａｌｌｔｉｍ ａ Ｃ１８色谱柱，以甲醇－０．０８ ｍ ｏｌ／Ｌ醋酸铵缓冲液（ｐＨ ６．０）（３２∶６８）为流动相，采用ＨＰＬＣ法测定石杉碱甲及其制剂的含量。理论板数按石杉碱甲峰计算大于３０００，最小检测量为０．２ ｎｇ （Ｓ／Ｎ≥３）。A RP HPLC method had been studied to determine the huperzine A and its preparations. An Alltima C 18 column (250×4.6mm ID) was used and the temperature of column was kept 60℃. The mobile phase was methanol 0.08 mol/L ammonium acetate buffer (pH 6.0) (32∶68) and the flow rate was 1.0 ml/min. The UV detection was performed at 308 nm. This method had a good linearity within the range of 0.02~0.64 (for dissolution test) and 3.0~7.0 μg/ml (for assay).

关 键 词：石杉碱甲 制剂 高效液相色谱 测定 

分 类 号：R971.4[医药卫生—药品] R927.2[医药卫生—药学]