不同时点应用维拉帕米对心肌缺血再灌注损伤的保护作用  

Protective effect of verapamil administered at different time points on myocardial ischemia-reperfu- sion injury in rats

作　　者：郑红升[1] 陈光献[1] 刘海[1] 黄邵洪[3] 戴刚[2] 吴钟凯[1] 

机构地区：[1]中山大学附属第一医院心脏外科,广州510080 [2]卫生部辅助循环重点实验室 [3]中山大学附属第三医院胸心外科

出　　处：《中华实验外科杂志》2011年第4期597-599,共3页Chinese Journal of Experimental Surgery

基　　金：国家重点基础研究发展计划(973计划)资助项目，国家杰出青年科学基金资助项目，国家自然科学基金资助项目

摘　　要：目的观察不同时点应用维拉帕米（VP）对大鼠心肌缺血再灌注损伤的保护作用，并探讨其心肌保护的作用机制。方法建立大鼠心肌缺血再灌注模型，将18只雄性SD大鼠随机分为3组，每组6只。Verapamil-1组于结扎前10rain开始泵入维拉帕米稀释液（0．25mg／kg），Verapamil-2组于再灌前10rain开始泵入维拉帕米稀释液（0．25mg／kg），IR组于结扎前10min开始泵人生理盐水（2ml／kg）。再灌注后60min处死大鼠。检测血清肌钙蛋白T（cTnT）含量、缺血区心肌组织Caspase-3表达水平；组织形态学分析心肌损伤程度。结果Verapamil-1组血清cTnT含量、心肌组织Caspase-3表达量（4．60±1．12）ng／L，（39．51±5．01）％较IR组（7．70±1．31）n∥L，（51．10±5．30）％和Vera—pamil-2组（7．23±1．03）ng／L，（49．35±4．95）％明显降低，差异有统计学意义（P〈0．05）；Verapamil-2组血清cTnT含量、心肌组织Caspase．3表达水平和IR组比较差异无统计学意义（P〉0，05）；Verapamil-1组心肌组织形态学损伤程度较IR组和Verapamil-2明显降低，差异有统计学意义（P〈0．05）；Verapamil-2组心肌组织形态学损伤程度和IR组比较差异无统计学意义（P〉0．05）。结论结扎前10rain开始给予维拉帕米对心肌缺血再灌注损伤有明显保护作用，再灌注前10min开始给予维拉帕米对心肌缺血再灌注损伤无保护作用。Objective To investigate the effect of verapamil administered at different time points on myocardial ischemia-reperfusion injury in rats, and explore the mechanism of myocardial protection. Methods The model of myocardial ischemia reperfusion in rats was established and 18 male SD rats were randomly divided into 3 groups, n = 6 each. Verapamil dilution （0. 25 mg/kg） was pumped into verapamil- 1 group 10 min before ischemia, and verapamil dilution （0. 25 mg/kg） was pumped into verapamil-2 group 10 min before reperfusion. Normal saline （2 ml/kg） was pumped into IR group 10 rain before ischemia. Rats were killed 60 min after reperfusion. The levels of serum cardiac Troponin T （cTnT） and the expres- sion of myocardial Caspase-3 were evaluated. Histomorphological methods were used to analyze the extent of myocardial injury. Results The levels of serum eTnT and the expression of myocardial Caspase-3 in verapamil-1 group （ 4. 60 ± 1.12 ） ng/L, （ 39.51 ± 5.01 ） % were significantly lower than those in IR group （7.70±1.31） ng/L, （51.10±5.30）% andverapamil-2 group （7.23 ±1.03） ng/L, （49.35± 4. 95 ） % （P 〈 0. 05 ）. The levels of serum eTnT and the expression of myocardial Caspase-3 had no signifi- cant difference between verapamil-2 group and IR group （ P 〉 0. 05 ）. The extent of myocardial injury in verapamil-1 group was significantly lower than that in IR group and verapamil-2 group （ P 〈 0. 05 ）. The ex- tent of myocardial injury had no significant difference between verapamil-2 group and IR group （P 〉 0. 05）. Conclusion Starting from 10 min before isehemia, verapamil has protective effects on myocardial isehemia/reperfusion injury. Starting from 10 rain before reperfusion, verapamil does not provide protection on myocardial ischemia reperfusion injury.

关 键 词：维拉帕米 心肌缺血 再灌注损伤 

分 类 号：R285.5[医药卫生—中药学]