白细胞介素-11类似物c(CGRRAGGSC)与人前列腺癌PC-3细胞的特异性结合研究  

Specific binding between IL-11 analogue c （ CGRRAGGSC ） and PC-3 cells in vitro

作　　者：杨泽萱[2] 刘璐[1] 吴清华[1] 朱晓莉[2] 

机构地区：[1]东南大学医学院核医学技术研究所,南京210009 [2]东南大学附属中大医院呼吸科

出　　处：《中华实验外科杂志》2011年第4期600-602,F0004,共4页Chinese Journal of Experimental Surgery

基　　金：基金项目：国家自然科学基金资助项目（30470500）

摘　　要：目的探讨白细胞介素-11（IL-11）类似物环九肽c（CGRRAGGSC）与人前列腺癌PC-3细胞的体外结合和在前列腺癌骨转移模型的体内分布。方法印迹蛋白分析PC一-细胞和正常前列腺细胞的IL-11受体（IL-11R）的表达。荧光检测c（CGRRAGGSC）与PC-3细胞的结合位点及内化。将28只荷瘤裸鼠随机分为7组，用99Tcm-标记c（CGRRAGGSC）制备99Tcm-DTPA．c（CGRRAGGSC），经尾静脉注射0．74MBq／0．2ml，观察标记物在荷瘤鼠体内的生物分布。结果PC-3细胞IL-11R是正常前列腺细胞的2．12倍。荧光示踪复染合成物结合在Pc-3细胞膜及胞质。标记物在模型体内迅速持续聚集在瘤体，4h瘤体、骨骼％ID／g达到峰值（15．12±1．19、7．03±1．83），其他脏器分布少，差异有统计学意义（F值依序为71．58、9．58，P〈0．05）。结论环九肽与PC-3细胞的结合具有特异性，标记物能与前列腺癌骨转移灶靶向结合。Objective To assess the specific binding between interleukin-ll （IL-11） analogue c （CGRRAGGSC） and PC-3 cells in vitro and biostribution in bone metastases of prostate cancer in mice in vivo. Methods The expression changes of IL-11R in PC-3 tumors and normal prostate cells were exam- ined by Western blotting. The binding site of the molecular target and internalization with PC-3 cells was observed under a fluorescence microscope. Twenty-eight tumor-bearing nude mice were divided into 7 groups, and radioactive molectdar probe 99Tcm-DT-PA- c （CGRRAG-GSC） was radiolabeled with 99Tcm. Biodistribution in nude mice bearing PC-3 tumors was observed and analyzed after intravenous injection of 99Tcm-DTPA-c （CGRRAGGSC） （0.74 MBq/0.2 ral）. Results The expression of IL-11R in PC-3 tumors group was 2. 2 times as the normal group. The counter stain molecular probe was combined with the PC-3 cell membrane and cytoplasm through fluorescence tracing. Uptake of the imaging probe in the tumor was increased. Radioactivity was abnormally concentrated in the tumor lesions and skeleton. Most apparent after 4 h （ 15.12 ± 1.19, 7.03 ± 1.83 ）, radiation was not obviously observed in other viscera （ F =71.58, 9. 58 ,P 〈 0. 05）. Conclusion c （CGRRAGGSC） peptide has the specific binding ability in targeting to PC-3 cells in vitro. Radioactive molecular probe 99Tcm-DTPA-c （CGRRAGGSC） has potential as a specific molecular target imaging agent for bone metastasis, such as prostate cancer.

关 键 词：多肽类 肿瘤/前列腺癌 

分 类 号：R737.25[医药卫生—肿瘤]