铅对小鼠肝脏超氧化物歧化酶活性及CuZn-SOD mRNA表达的影响  

EFFECT OF LEAD ACETATE ON ACTIVITY OF SUPEROXIDE DISMUTASE AND CUZN-SOD MRNA EXPRESSION IN RATS' LIVER

作　　者：梁建成[1] 邓树嵩[1] 马迎教[1] 李韬[2] 

机构地区：[1]右江民族医学院预防医学系,百色533000 [2]右江民族医学院基础医学院生物化学教研室

出　　处：《现代预防医学》2011年第7期1220-1222,共3页Modern Preventive Medicine

基　　金：广西壮族自治区教育厅科研项目(2006214)

摘　　要：[目的]以醋酸铅染毒小鼠为模型,研究铅对肝脏超氧化物歧化酶(SOD)活性及CuZn-SODmRNA表达的影响。[方法]分别以浓度为0、0.1%、0.2%、0.4%醋酸铅喂养小鼠。6周后取肝脏,试剂盒测定SOD活性;RT-PCR法测定CuZn-SODmRNA表达水平。[结果]与对照组比较,染毒组SOD活性明显下降(P﹤0.01),CuZn-SODmRNA表达显著下调(P﹤0.01),且两指标均存在剂量-效应关系(r=0.901,P﹤0.01)。[结论]铅可致小鼠肝脏SOD活性下降,CuZn-SODmRNA表达下调,两者存在良好效应关系,呈正相关。[Objective]To study the effect of lead acetate on activity of superoxide dismutase and CuZn-SOD mRNA expression in mice＇s hepatocytes.[Methods]32 male KM rats were randomly divided into 4 groups.Each group received lead acetate at random in drinking water at a concentration of 0,0.1%,0.2% or 0.4% for 6 weeks,respectively.The activity of SOD in liver was detected,and the expression of CuZn-SOD mRNA was determined by RT-PCR.[Results]Compared with the control group,the activity of SOD and the expression of CuZn-SOD mRNA of experimental groups were both decreased significantly（P﹤0.01）,they also presented significant dose-effect relationship（P﹤0.01）.[Conclusion]The activity of SOD and the expression of CuZn-SOD mRNA are depressed by lead acetate in rats＇ liver.

关 键 词：醋酸铅 超氧化物歧化酶 CuZn-SODmRNA 

分 类 号：R135.11[医药卫生—劳动卫生]