骆驼蓬总RNA的4种提取方法比较  

COMPARISON OF FOUR METHODS FOR EXTRACTING TOTAL RNA FROM Peganum harmala L.

作　　者：石庆华[1] 代培红[1] 许磊[1] 

机构地区：[1]新疆农业大学农业生物技术重点实验室,乌鲁木齐830052

出　　处：《内蒙古农业大学学报（自然科学版）》2010年第4期152-155,共4页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：新疆维吾尔自治区科技厅重点实验室开放项目(XJYS0302-2009-01)

摘　　要：以骆驼蓬(Peganum harmala L.)为材料,采用植物总RNA提取试剂盒法(TIANGEN)、TRIZOL试剂盒法(TIANGEN)、SDS-异丙醇法、CTAB-LiCl法提取总RNA,比较4种方法提取总RNA的得率和纯度。结果表明:CTAB-LiCl法效果最好,电泳条带清晰,RNA完整性好,28S条带亮度约是18S的2倍,所提RNA的OD260/OD280的值在1.80～2.0之间。经RT-PCR获得了600bp大小的特异条带,说明利用改良的CTAB-LiCl法从骆驼蓬中提取到的RNA质量高、完整性好,完全适合于进一步的分子生物学研究。Several commercialized or commonly used methods,such as RNA Plant Reagent（Tiangen）,TRIZOL Reagent（Tiangen）,SDS-Isopropanol method,CTAB-LiCl method were used to isolate RNA from leaves of wild Peganum harmala L..Comparing quality and purity.The results showed high purity and integrated RNA was obtained by CTAB-LiCl method with no degradation or contaminant,the 28S and 18S bands in agarose gel electrophoresis were clear,and the brightness ratio of two bands（28S/18S） was more than 2.0,the ratio of A260/A280 was between 1.80 and 2.0.600bp gene fragments were successfully amplified by RT-PCR,suggesting the high integrity of isolated RNA.The total RNA isolated by CTAB-LiCl method was sufficient quality for subsequent molecular biology researches.

关 键 词：骆驼蓬 总RNA提取 SDS-异丙醇法 CTAB-LiCl法 

分 类 号：Q949.752.6[生物学—植物学]