甲酚红褪色分光光度法测定氯诺昔康  

FADING SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF LORNOXICAM WITH GESOL RED

作　　者：阿娟[1] 刘惠民[1] 敖登高娃[2] 金迎春[2] 

机构地区：[1]内蒙古农业大学理学院,呼和浩特010018 [2]内蒙古大学化学化工学院,呼和浩特010021

出　　处：《内蒙古农业大学学报（自然科学版）》2010年第4期276-279,共4页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

摘　　要：在pH8.75的tris-HCl缓冲溶液中,甲酚红与氯诺昔康反应形成离子缔合物,使甲酚红溶液褪色。实验结果表明:最大褪色波长位于571nm,氯诺昔康浓度在0.074 36μg/mL～14.87μg/mL范围内遵循比尔定律,回归方程为ΔA=0.132 6c-0.021 3(c=μg/mL),相关系数为r=0.999 3,表观摩尔吸光系数为4.824×104 L.mol-1.cm-1。据此建立了测定生物样品中氯诺昔康含量的褪色分光光度法,样品测定平均回收率为98.46%～100.8%。In pH 8.75 tris-HCl buffer medium,Cresol Red reacted with Lornoxicam to form an ion association complex,which causes the fading of the Cresol Red solution.The results showed that the maximum fading wavelength is located at 571nm.The concentration of Lornoxicam obeyed the Beer′s law in the range of 0.07436~14.87μg/mL with the regression equation wasΔA=0.1326c-0.0213（c=μg/mL）.The correlation coefficient was 0.9993,and the apparent molar absorptivity was 4.824×104 L·mol-1·cm-1.Fading spectrophotometric method has been developed for the determination of lornoxicam in biological samples with Lornoxicam.The recovery of sample was within the the range of 98.46%~100.8%.

关 键 词：氯诺昔康 甲酚红 褪色分光光度法 

分 类 号：O657.3[理学—分析化学]