Sequencing and Analysis of ITS Sequences of Bangia atropurpurea  

暗紫红毛菜ITS序列的测定与分析(英文)

作　　者：李峰[1] 冯佳[1] 谢树莲[1] 

机构地区：[1]山西大学生命科学学院,山西太原030006

出　　处：《Agricultural Science & Technology》2010年第9期45-46,192,共3页农业科学与技术（英文版）

基　　金：Supported by National Science Foundation of China(30970187)~~

摘　　要：[Object]To providing new data for phylogenesis of Bangia atropurpurea by the sequencing of internal transcribed spacer（ITS region）of rDNA.[Method]B.atropurpurea was collected from Niangziguan Spring in Shanxi,the DNA was extracted and the primers were designed for PCR amplification so as to obtain ITS gene sequence.[Result]The homology of ITS region between B.atropurpurea and Porphyra oligospermatangia was 75%,which was 79% between B.atropurpurea and P.yezoensis.[Conclusion]Compared with other genes,the ITS sequence had a greater evolution rate.The differences in classification and distribution resulted in the sequence diversity.[目的]对暗紫红毛菜rDNA内转录间隔区(ITS区)进行序列测定,为其系统发育研究提供新资料。[方法]以产于山西娘子关泉的一株暗紫红毛菜为材料,提取DNA,设计引物,进行PCR扩增,进而测定其ITS区基因序列。[结果]暗紫红毛菜与同科的少精紫菜ITS区同源性为75%,与条斑紫菜ITS区同源性为79%。[结论]ITS区序列进化速率相对较快,种类和地理分布的差异是其序列差异产生的原因。

关 键 词：Bangia atropurpurea ITS1-5.8SrDNA-ITS2 SEQUENCE 

分 类 号：Q943.2[生物学—植物学]