Application of Plant Growth Regulator TDZ in Strawberry Anther Culture  

植物生长调节剂TDZ在草莓花药培养中的应用(英文)

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作  者:王敬东[1] 张丽[1] 马洪爱[1] 陈晓军[1] 宋玉霞[1] 

机构地区:[1]宁夏农林科学院农业生物技术研究中心,宁夏银川750002

出  处:《Agricultural Science & Technology》2010年第9期82-84,90,共4页农业科学与技术(英文版)

基  金:Supported by Science and Technology Research Projects in Ningxia Hui Autonomous Region(KGZ-16-07-02);Opening project in National Engineering Laboratory of Tree Breeding in Beijing Forestry University~~

摘  要:[Objective] The aim was to investigate the application of plant growth regulator thidiazuron(TDZ)in strawberry anther culture.[Method]Different combinations of TDZ and naphthalene acetic acid(NAA)auxine with different concentrations were used for induction culture,and then its effect on callus induction and plant regeneration were observed.[Result] The combination of 1.0 mg/L TDZ+0.5 mg/LNAA was effective to accelerating the differentiation and regeneration of strawberries,and the differentiation ratio was as high as 75%.However,TDZ showed insignificant effect on strawberries anther induction.[Conclusion]This research had provided reference for large-scale production of strawberry through anther culture.[目的]探讨植物生长调节剂TDE在草莓花药培养中的应用。[方法]采用不同浓度TDZ与NAA植物生长素进行组合,观察其对草莓愈伤组织诱导及其分化的影响。[结果]MS+TDZ1.0mg/L+NAA0.5mg/L适用于草莓愈伤组织的分化,分化率高达75%,但TDZ对草莓愈伤组织的诱导无显著作用。[结论]该研究结果为草莓通过花药培养途径进行规模生产提供参考。

关 键 词:Strawberry anther TDZ CALLUS Adventitious bud 

分 类 号:S668.4[农业科学—果树学]

 

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