Germination Characteristics and Secondary Metabolism Regulation of Scutellaria baicalensis Georgi Seeds under Different Temperatures  

温度对黄芩种子发芽特性和次生代谢的调节(英文)

作　　者：刘金花[1] 李佳[1] 张永清[1] 

机构地区：[1]山东中医药大学药学院,济南250355

出　　处：《Agricultural Science & Technology》2010年第9期213-216,共4页农业科学与技术（英文版）

基　　金：Supported by Technology Development Plan of Shandong Province(2008GG2NS02022);Agricultural Seed Project in Shandong Province(2009LZ01-03);National Key Basic Research Program(2007CB512601)~~

摘　　要：[Objective] To study the germination characteristics and secondary metabolism of Scutellaria baicalensis Georgi seeds under different temperatures.[Method]The activities of superoxide dismutase（SOD）and peroxidase（POD）were determined with lactoflavine-NBT method and guaiacol-colorimetry assay,respectively.The catalase（CAT）,ascorbate peroxidase（APX）,phenylalanine ammonia-lyase（PAL）and cinnamic acid 4-Hydroxylase（C4H）were determined with ultraviolet spectrophotometry.The secondary metabolites were detected by High Performance Liquid Chromatography（HPLC）.[Result]The germination percentage,germination potentiality and germination index were seriously affected by low or high temperature.The proper germination temperature was 20-25 ℃.The activities of SOD,POD and CAT were significantly decreased as comparison to suitable temperature.The activities of PAL and C4H were also significantly decreased as comparision to suitable temperature which was accordance to the secondary metabolites.There was a positive correlation between the flavonoids content and the PAL and C4H activity（r=0.956,r=0.951,P0.05）.[Conclusion]The quality of the skullcaps could be improved by improving the activity of PAL and C4H.The proper temperature for the seed germination and the formation of secondary metabolites was 20 ℃.[目的]研究不同温度对黄芩种子发芽特性和次生代谢的调节。[方法]超氧化物岐化酶(SOD)活性测定采用核黄素-NBT法,过氧化物酶(POD)活性测定采用愈创木酚比色法,过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、苯丙氨酸解氨酶和肉桂酸-4-羟化酶活性测定采用紫外分光光度法。次生代谢物质含量测定采用高效液相色谱法。[结果]黄芩种子的发芽率、发芽势和发芽指数均受温度影响。黄芩种子的最适发芽温度为20-25℃;与适温相比,在低温和高温下SOD,POD和CAT的活性明显降低,PAL和C4H也呈现相类似的变化趋势,这与其次生代谢物质的变化也相同;次生代谢物质(黄酮类化合物)与其代谢途径中的关键酶(PAL和C4H)具有良好的相关性(r=0.956,r=0.951,P<0.05)。[结论]黄芩种子发芽以及次生代谢物质合成的适宜温度为20℃。黄芩药材中次生代谢物质的含量可通过提高PAL和C4H的活性来提高。

关 键 词：Germination characteristics Antioxidant enzyme PAL C4H Secondary metabolites 

分 类 号：S567.239[农业科学—中草药栽培]