人IL-21基因联合γ射线照射对子宫颈癌Hela细胞生长的影响  

Effects of Adenovirus-mediated IL-21 Gene Vector with γ-ray Radiation on Growth of Cervical Carcinoma Cells

作　　者：邵盈[1] 曹永珍[1] 王芹[2] 李进[2] 赵欣然[2] 陈凤华[2] 宋力[2] 张鸿艳[1] 

机构地区：[1]天津医科大学总医院放疗科,天津300052 [2]中国医学科学院放射医学研究所天津市分子核医学重点实验室,天津300192

出　　处：《中国辐射卫生》2011年第1期15-16,共2页Chinese Journal of Radiological Health

基　　金：天津市自然科学基金项目(09JCYBJC09300);中国医学科学院放射医学研究所探索基金(ST1006)

摘　　要：目的研究含人IL-21基因的重组腺病毒(Ad-IL-21)联合γ射线照射对子宫颈癌Hela细胞生长的抑制作用。方法重组腺病毒Ad-IL-21于体外转染子宫颈癌Hela细胞,转染后6 h进行6Gy 137Csγ射线照射,用MTT方法和流式细胞仪测定Hela细胞的生长曲线、抑制率及细胞周期。结果 Ad-IL-21基因组、照射组和基因联合照射组对Hela细胞生长均具有抑制作用,联合照射组的抑制效应最强(44%),明显高于Ad-IL-21组和照射组。联合照射组Hela细胞出现明显的G1期阻滞,G1期细胞所占比例最高,达到88.9%,S期细胞最少(1%)。结论 IL-21基因联合γ射线照射对子宫颈癌的抑瘤作用具有协同效应,有效地抑制肿瘤细胞的生长。Objective To study the effect of inhibition on the growth of cervical carcinoma cells by introduction of foreign IL -21 gene vector with γ, - ray. Methods The recombinant IL -21 gene adenovirus vector was transfected into cervical carcinoma cell line Hela . Hela cells were irradiated with 6Gy137Cs γ, - ray after 6 hours transfection The cells growth curve and cells cycle of Hela cells were detected by MTI＂ assay and flow cytometry respectively. Results The growth of Hela cells transfected with Ad - IL - 21, radiation and Ad - IL - 21 combined with radiation group was all inhibited. The group of Ad- IL- 21 combined with radiation resulted in greater inhibition of cells growth, reaching 44% , compared with Ad- IL- 21 infection and radiation group. Cells of G1 phases of Hela cells for Ad - IL - 21 combined with radiation group were the highest, reaching 88.9% , while those of S phases were the lowest, reaching 1%. Conclusion Ad - IL -21 gene transfer combined with radiation show synergism for the inhibition of cervical cancer Hela cells growth.

关 键 词：IL-21基因 子宫颈癌 腺病毒 照射 

分 类 号：Q691[生物学—生物物理学]