人白介素21基因联合γ射线照射对食管癌EC109细胞周期的影响  

Effect of Human Interleukin-21 Gene Transfer Combined with γ-ray Radiation on Cell Cycle of Esophageal Cancer Cells

作　　者：张鸿艳[1] 赵欣然[2] 曹永珍[1] 李进[2] 王芹[2] 陈凤华[2] 宋力[2] 邵盈[1] 

机构地区：[1]天津医科大学总医院放疗科,天津300052 [2]中国医学科学院放射医学研究所天津市分子核医学重点实验室,天津300192

出　　处：《中国辐射卫生》2011年第1期37-39,共3页Chinese Journal of Radiological Health

基　　金：天津市自然科学基金资助项目(09JCYBJC09300);中国医学科学院放射医学研究所探索基金资助(ST1006)

摘　　要：目的研究含有IL-21基因的重组腺病毒表达载体(Ad-IL-21)联合γ射线照射对食管癌细胞EC109生长的抑制作用,为肿瘤的基因-放射治疗提供实验依据。方法将Ad-IL-21于体外转染食管癌细胞株EC109,用MTT比色法和流式细胞术观察基因联合照射后EC109细胞的生长曲线和细胞周期的变化。结果 Ad-IL-21基因组、单纯照射组和Ad-IL-21基因联合照射组EC109细胞生长均明显受到抑制,与Ad-lacZ组和空白对照组比较差异均有统计学意义(P<0.05)。其中联合照射组EC109细胞生长最为缓慢,与Ad-IL-21基因组和单纯照射组比较差异均有统计学意义(P<0.05)。Ad-IL-21基因组、单纯照射组和联合治疗组大量的EC109细胞停留在G1期,而G2期和S期细胞明显减少,联合治疗组细胞停留在G1期最多。结论腺病毒介导的白介素21基因转移能显著抑制食管癌EC109细胞的生长,使细胞产生G1期阻滞,与放射治疗联合应用具有相加和协同效应。Objective To study the combined effect of adenovirus -mediated IL -21 gene and ionizing radiation on the growth of esophageal cancer cells, and explore a gene therapy approach for esophageal cancer. Method The esophageal cancer cell line ECI09 was transfected by recombinant IL - 21 gene adenovirus vector and irradiated by 6 Gy γ- rays after transfection. The cohorts were divided into blank control ,Ad- lacZ ,Ad- IL- 21 ,irradiation and Ad- IL- 21 combined with irradiation. The cell growth curve and celt cycle of EC109 cell was detected by M＇IT assay and flow cytometry respectively. Results The growth of EC109 cells transfected with Ad - IL - 21, radiation and combination of Ad - IL - 21 and radiation was slower than that of Ad - lacZ and blank control significantly（ P 〈 0.05 ）. The combination of Ad - IL - 21 and radiation resulted in greater inhibition of cell growth compared with Ad- IL- 21 infection and radiation（P 〈0.05）. Cells of G1 phase transfected with Ad -IL- 21 and radiation increased, cells of G2 and S phase decreased. Cells of the combination of Ad -IL - 21 and radiation were arrested at G1 phases of cell cycle mainly. Conclusion Adenovirus - mediated IL - 21 gene can in- hibit ECI09 cell growth and arrest the cells at G1 phase. It demonstrates a combined effect of Ad -IL- 21 gene transfer and radiation in esophageal cancer.

关 键 词：IL-21基因 放射 食管癌 腺病毒载体 

分 类 号：Q691[生物学—生物物理学]