莽山烙铁头蛇粗毒双向凝胶电泳分析  

Analysis of Two-Dimensional Electrophoresis of Toxin From the Ermia mangshanensis

作　　者：聂东宋[1] 刘宇[1] 吴喆[1] 刘立超[1] 朱政斌[1] 

机构地区：[1]湖南理工学院化学化工学院,湖南岳阳414006

出　　处：《湖南理工学院学报（自然科学版）》2011年第1期48-51,共4页Journal of Hunan Institute of Science and Technology(Natural Sciences)

基　　金：湖南省教育厅科研项目(06C370);湖南省教育厅青年基金项目(07B029);湖南省高校科技创新团队支持计划资助项目

摘　　要：采用固相pH梯度等电点聚焦-SDS双向聚丙烯酰胺凝胶电泳对莽山烙铁头粗毒进行了分析,通过银染法显色,电脑软件识别出约40个蛋白质点.其中分子量超过130 kD的蛋白质点很少,分子量90 kD^10 kD的区域均有蛋白质点分布,分子量34 kD区域的蛋白质点较为密集,且它们的含量明显较高.从蛋白质点的等电点分布范围分析,偏于碱性端的蛋白质明显多于酸性端的蛋白质,且分布在中性偏碱(pH 7~8)的区域.质谱数据的数据库搜寻结果表明:莽山烙铁头蛇毒中含有类凝血酶组分、纤溶酶组分、血小板聚集蛋白、抗血小板聚集蛋白和平滑肌钙离子通道阻断剂等组分.In this study,Crude venom of the Ermia mangshanensis snake was separated by two-dimensional gel electrophoresis（2D-PAGE） with the separation in the first dimension on a wide range immobilized pH（3-10） gradient.The results showed that over 40 protein spots were presented on a silver stained 2D-gel.Molecular weight More than 130 kD were almost not detected.About 35 spots with relatively high concentration were located on the region among the molecular weight of 34 kD.The basic proteins are much more than the Acidic proteins within the range from pH 7～8.MS database search results showed that Ermia mangshanensis venom contained thrombin,plasmin components,platelet aggregation protein,anti-platelet aggregation protein,smooth muscle components of calcium channel blocking agents.

关 键 词：莽山烙铁头蛇毒 二维电泳 蛋白质组 

分 类 号：Q786[生物学—分子生物学]