杏花雨注射液对荷瘤小鼠血清IL-2、TNF-α水平的影响  

Effects of Xinghuayu injection on IL-2 and TNF-α level in serum of tumor-bearing mice

作　　者：徐月清[1] 许光辉[2] 牛培[1] 巴伟松[2] 王晶[3] 杜宁[4] 李利平[1] 李娟 王乾[1] 

机构地区：[1]河北大学医学部,河北保定071000 [2]定州市人民医院,河北定州071012 [3]中国人民解放军第252医院,河北保定071000 [4]保定市妇幼保健院,河北保定071000 [5]北京万金阳光医药科技有限公司,北京100038

出　　处：《医学研究与教育》2011年第1期5-7,共3页Medical Research and Education

基　　金：河北省科技厅课题(062761399)

摘　　要：目的观察杏花雨注射液对肝癌H22模型小鼠血清中IL-2及TNF-α水平的影响。方法将接种肝癌H22瘤细胞的昆明鼠随机分为5组,即模型对照组、杏花雨注射液低剂量组、杏花雨注射液中剂量组、杏花雨注射液高剂量组及环磷酰胺阳性对照组。于接种后24h各组开始给药,连续7d。末次给药后24h,内眦取血测定血清IL-2及TNF-α浓度。结果杏花雨注射液中、高剂量组血清IL-2浓度明显高于模型对照组(P<0.05);杏花雨注射液中、高剂量组血清TNF-α浓度明显高于模型对照组(P<0.01)。结论杏花雨注射液能提高荷瘤小鼠血清IL-2及TNF-α水平。Objective To observe the effects of Xinghuayu injection on IL-2 and TNF-α level in serum of tumor-bearing mice.Methods Fourty Kunming mice were randomly divided into 5 groups： model group,three dose Xinghuayu injection groups and cyclophosphamide group.And every mice was subcutaneously injected with hepatoma（H22） cell suspension,and 24 hours later the intraperitoneal injection was done one time a day and it lasted for 7 days.At 24 hours after the last injection,the blood was got in the eyes bare.The serum concentration of IL-2 and TNF-α was determined.Results The serum concentration of IL-2 in both middle and high dose groups of Xinghuayu injection was significantly higher than that of model group（P〈0.05）;The serum concentration of TNF-α in both middle dose and high dose groups of Xinghuayu was significantly higher than that of model group（P〈0.01）.Conclusion Xinghuayu injection can enhance the serum level of IL-2 and TNF-α of tumor-bearing mice.

关 键 词：杏花雨注射液 肝癌H22细胞 IL-2 TNF-Α 

分 类 号：R96[医药卫生—药理学]