采用胸导管结扎术建立胰淋巴淤滞实验动物模型的观察  

Establishment of experimental animal model with pancreatic lymph stagnation induced by ligaturing thoracic duct in rats

作　　者：何海燕[1,2] 史树堂[1,3] 王保芝[1] 赵宇清[1] 刘子宸[1] 

机构地区：[1]河北医科大学基础医学院人体解剖学教研室,河北石家庄050017 [2]河北体育学院,河北石家庄050041 [3]河北大学基础医学院,河北保定071000

出　　处：《医学研究与教育》2011年第1期8-11,22,共5页Medical Research and Education

基　　金：河北省自然科学基金(C2007000823)资助项目;河北省教育厅博士基金(B2004-125)资助项目

摘　　要：目的建立淋巴回流阻滞的实验动物模型,并通过形态学观察进行评价。方法 10月龄SD大鼠20只,随机分为两组,实验（手术）组15只和对照（假手术）组5只;术前30~60min用植物油2－5mL灌胃,经6%水合氯醛（5mL/kg）腹腔注射麻醉。在手术显微镜下,对照组只游离胸导管而不结扎。实验组在充分暴露胸导管的基础上,于腹部膈脚下将其结扎并进行观察。术后6个月在深度麻醉下切取大鼠胰组织标本,行石蜡包埋和组织切片,常规HE和刚果红染色后,进行光镜观察。结果肉眼可见,实验动物的胸导管被结扎后,可见乳糜池出现充盈,远端淋巴干和淋巴管明显扩张。HE染色组织切片光镜观察显示,实验组与对照组相比,胰腺小叶间隙显著增宽,呈现明显的淋巴管扩张、结缔组织增生和脂肪堆积。实验组动物的胰组织切片刚果红染色观察提示,除了胰腺小叶间隙明显增宽,胰岛还普遍表现为朱红色着色现象。结论胸导管结扎可导致胰淋巴回流障碍,引起胰淋巴淤滞、淋巴管扩张、结缔组织间隙增宽、脂肪堆积及淀粉样物质沉积等。采用腹部结扎大鼠胸导管的方法,可以建立胰淋巴淤滞的实验动物模型,为探讨淋巴淤滞对胰组织结构、内分泌及代谢的影响,奠定了基础。Objective To establish the lymph drainage block animal model and observe the morphological changes of pancreatic tissue in fine structural level.Methods 20 adult SD rats at ten months were divided into 2 groups randomly,as experimental（operation） group and control（sham operation） group.The rats were sacrificed at 6 months after operation,the pancreatic tissue paraffin sections was stained with conventional HE and Congo red and observed with light microscope.Results Macroscopic observation： just after operation of thoracic duct ligation in rats,the cisterna chylimay were engorged with chyle,those far-end lymphatic trunks and lympgatic vessels were broadened obviously.Light microscope observation：the conventional HE staining of pancreatic tissue sections in experimental group showed that the pancreatic glandular lobule interstitial space were both widen,connective tissue desmoplasia,fatty cumulated,pancreatic islet light coloured.The Congo red staining sections in experimental group appeared that the pancreatic glandular lobule interstitial space was widen,pancreatic islets were stained with cinnabar red colour.Conclusion The morphological changes of rat pancreatic tissue structure after operation indicate that the ligation of thoracic duct may induce pancreatic lymph drainage block,lymphagiectasis,connective tissue interstitial space widen,fatty cumulating and amyloidal substance deposited.These structural changes may affect the endocrine function of pancreatic islet.

关 键 词：胰组织 胸导管 淋巴淤滞 

分 类 号：R544.1[医药卫生—心血管疾病]