天津地区番茄黄化曲叶病毒DNA-A的克隆和序列分析  

Cloning and Sequence Analysis of TYLCV DNA-A in Tianjin

作　　者：金凤媚[1] 薛俊[1] 郏艳红[1] 周祥明[1] 王建江[2] 刘仲齐[1] 

机构地区：[1]天津市农业生物技术研究中心,天津300192 [2]新疆石河子蔬菜研究所,新疆石河子832000

出　　处：《华北农学报》2011年第1期58-62,共5页Acta Agriculturae Boreali-Sinica

基　　金：国家科技支撑计划项目(2006BAD01A7-3-10);天津市农业科技成果转化项目(08ZHZNC03900)

摘　　要：对天津发生的番茄黄化曲叶病毒进行了分子鉴定,并对DNA-A的全基因组进行了序列分析。结果表明,天津分离物与国内山东、安徽、上海和浙江等地的分离物及亚洲(韩国、日本、约旦、以色列)和北美洲(美国、墨西哥)分离物的同源性为93%以上;与科摩洛、马达加斯加等非洲国家的同源性为80%。天津地区的分离物与广东G3分离物和广西的分离物不是同一病毒。编码AC2蛋白的开放阅读框有2处碱基发生突变,导致氨基酸90位E→G和104位H→Y。Tomato yellow leaf curl virus（TYLCV）isolate in Tianjin was identified at molecular level,and the genome sequence of DNA-A has been cloned and analyzed.The results showed that the homology of the isolate in Tianjin was 93%or more with the isolates in Shandong,Anhui,Shanghai,Zhejiang,South Korea,Japan,Jordan,Israel USA and Mexico.And the homology was 80%with Comoros,Madagascar and other African countries.The isolate in Tianjin was not the same virus with the isolates in Guangdong G3 and Guangxi.There were two bases in the open reading frame which encode AC2 protein occurred mutation,leading to the 90st amino acid of AC2 protein E→G and the 104st H→Y.

关 键 词：番茄黄化曲叶病毒 克隆 序列分析 

分 类 号：Q78[生物学—分子生物学]