四川白鹅PRL基因的克隆与生物信息学分析  

Cloning and Bioinformatic Analysis of the Prolactin Gene of Sichuan White Goose

作　　者：吴慧英[1] 刘铀[2] 贾汝敏[1] 张丽[1] 

机构地区：[1]广东海洋大学动物科学系,广东湛江524088 [2]广东海洋大学动物医学系,广东湛江524088

出　　处：《华北农学报》2011年第1期105-111,共7页Acta Agriculturae Boreali-Sinica

基　　金：湛江市科技招标项目(0609135);广东海洋大学基金项目(C04095);校企合作项目(0810314)

摘　　要：为了探明鹅就巢性产生的分子机理及不同鹅种之间的遗传分化,采用RT-PCR方法从四川白鹅垂体组织克隆到了PRL基因,并对其核苷酸序列和推导的氨基酸序列等进行了分析。四川白鹅PRL基因编码区含有690个核苷酸(Gen-Bank NO.GQ202542),编码229个氨基酸,与其他禽类PRL具有高度保守性;其中与东北白鹅和籽鹅的PRL基因核苷酸序列同源性最高,达到100%;与莱茵鹅、马岗鹅、皖西白鹅、浙东白鹅同源性达到99.4%～99.9%;与北京鸭和番鸭相比分别为98.6%和98.4%;与鹌鹑、家鸡、火鸡的同源性介于92.3%～93%。四川白鹅PRL蛋白二级结构由多个α螺旋和β转角及无规卷曲构成,在其胞外区有2个糖基化位点,推测N端70～76、95～102、150～155和207～213区段为其抗原表位优势区。四川白鹅及其他鹅种PRL氨基酸序列具有一个潜在信号肽剪切位点(28VTS-LP32),此位点的差异可能导致PRL前体翻译加工的不同,从而引起禽种在就巢行为上的差异。For further study of the molecule mechanism of broodiness and the heredity differentiation among geese,the prolactin（PRL） cDNA gene was amplified by RT-PCR and then cloned from anterior pituitary gland of Sichuan White Goose,then the bioinformatics of the sequences were analysed.The results showed that the PRL coding region comprised 690 nucleotides（GenBank NO.GQ202542）and encoded a putative protein of 229 amino acids,which has 100% homology with Zi goose and Dongbei White goose,Shared 99.4%-99.9% with Rhine,Magang,Wanxi White and Zhedong White goose.Compared with Peking duck and Muscovy are 98.6% and 98.4% respectively,and 92.3%-93% homology with quail,chicken,and turkey.From the protein form,it found that the PRL protein comprised of several α-helixes,β-sheets and coils,with 2 N-glycosylation site.Infered that the immunodominant epitope were located in the N′ 70-76,95-102,150-155 and 207-213 sections.It was found that the Sichuan White goose and other kind of geese had a signal peptide cleavage site（28VTS-LP32）.Such difference might cause a different translation processing of pre-prolactin,which could make the different goose broody behaviors.

关 键 词：四川白鹅 催乳素(PRL)基因 克隆 生物信息学分析 

分 类 号：S835[农业科学—畜牧学] Q785[农业科学—畜牧兽医]