高效液相色谱法测定泽虎降脂胶囊中大黄素含量  

Determination of Emodin in Zehu Jiangzhi Capsules by HPLC

作　　者：王儿 

机构地区：[1]浙江省嵊泗中医院药剂科,浙江舟山316000

出　　处：《中国药业》2011年第7期17-18,共2页China Pharmaceuticals

摘　　要：目的建立测定泽虎降脂胶囊中大黄素含量的高效液相色谱法。方法色谱柱为Alltima C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-0.1%磷酸溶液(85∶15),流速为1.0mL/min,检测波长为287nm,柱温为30℃。结果大黄素进样量在0.0890～0.8900μg范围内与峰面积线性关系良好,r=0.9999(n=5);平均加样回收率为98.04%,RSD为1.91%(n=6)。结论该方法简便、准确、重现性好,可用于泽虎降脂胶囊的含量测定。Objective To establish the determination method of emodin in Zehu Jiangzhi Capsules by HPLC.Methods HPLC was adopted with Alltima C18 column（250 mm×4.6 mm,5μm）at a temperature of 30℃.The mobile phase consisted of methanol-0.1%hosphoric acid（85∶15）with a flow rate of 1.0 mL/min.AUV-detector at 287 nm was used,column temperature was 30℃.Results Emodin showed good linear relationship in the range of 0.089 0-0.890 0μg,r=0.999 9（n=5）.The average recovery rate was 98.04%,RSD=1.91%（n=6）. Conclusion The method is simple and accurate with a good reproducibility,it can be used for the quality control of Zehu Jiangzhi Capsules.

关 键 词：高效液相色谱法 泽虎降脂胶囊 大黄素 含量测定 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]