紫外分光光度法测定丁卡因氯己定酊中两组分含量  

Determination of the Content of Tetracaine and Chlorhexidine Tincture by UV Spectrophotometry

作　　者：郑维国[1] 彭其胜[1] 

机构地区：[1]重庆市涪陵中心医院药剂科,重庆408000

出　　处：《中国药业》2011年第7期31-32,共2页China Pharmaceuticals

摘　　要：目的建立丁卡因氯己定酊的含量测定方法。方法用紫外分光光度法直接测定盐酸丁卡因含量,测定波长为310 nm;用系数倍率法测定醋酸氯己定含量,测定波长为254 nm,转换波长为310 nm。结果盐酸丁卡因质量浓度在2.71～10.82μg/mL范围内与吸光度线性关系良好,r=0.9999(n=7),平均回收率为99.91%,RSD为0.58%(n=6);醋酸氯己定质量浓度在2.51～10.04μg/mL范围内与吸光度线性关系良好,r=0.9995(n=7),平均回收率为99.76%,RSD=0.93%(n=6)。结论所用方法简便、快速、准确,适宜于该制剂的快速检验。Objective To establish an assay method for determination of Tetracaine and Chlorhexidine Tincture.Methods The content of tetracaine hydrochloride was detemined directly at 310 nm by UV spectrophotometry;the content of chlorhexidine acetate was detemined at 254 nm by K-ratio spectrophotomerty,transform wavelength is 310 nm.Results The calibration curve for tetracaine hydrochloride was linear within the range of 2.71-10.82μg/mL,r=0.999 9（n=7）,the average recovery rate was 99.91%（n=6）,and the RSD was 0.58%;the calibration curve for chlorhexidine acetate was linear within the range of 2.51-10.04μg/mL,r=0.999 5（n=7）,the average recovery was 99.76%（n=6）,and the RSD was 0.93%.Conclusion The method is simple,rapid and accurate,and suitable for rapid assay of the preparation.

关 键 词：紫外分光光度法 系数倍率法 丁卡因氯己定酊 盐酸丁卡因 醋酸氯己定 含量测定 

分 类 号：R927.2[医药卫生—药学] R982