高晶体-高胶体渗透压溶液对受机械性损伤的海马神经细胞容积的影响  

The Effect of Hypertonic Hyperoncotic Solution on Cell Volume of Mechanical Damaged Hippocampal Neurons in Primary Culture

作　　者：袁世荧[1] 姚尚龙[1] 毛卫克[1] 曾邦雄[1] 

机构地区：[1]同济医科大学附属协和医院麻醉科,武汉430022

出　　处：《同济医科大学学报》1999年第4期317-319,共3页Acta Universitatis Medicinae Tongji

摘　　要：观察机械性损伤的海马神经细胞在不同浓度高晶体－ 高胶体渗透压溶液中容积的变化。取原代培养大白鼠胚胎的海马神经细胞，用超声波机械损伤细胞后暴露于含０．５ ｇ／Ｌ、１ ｇ／Ｌ和２．５ ｇ／Ｌ氯化钠的细胞培养基中１５ ｍ ｉｎ。结果显示机械性损伤的细胞明显肿胀（Ｐ＜ ０．０５）。当细胞暴露于不同浓度的高晶体－ 高胶体溶液１５ ｍ ｉｎ 后， 细胞容积与同一实验时间的对照值相比明显下降， 并保持至第７ ｄ （Ｐ＜ ０．０５）。表明受到机械性损伤的海马神经细胞在高晶体－ 高胶体渗透压环境中的容积调节功能丧失或减弱。The changes of cell volume in mechanical damaged hippocampal neurons of rat embryo in vitro were observed after exposure to different concentrations of hypertonic hyperoncotic solutions Primary cultured hippocampal neurons of rat embryo was damaged by ultrasound and exposed to cell medium with concentrations of 0 5 g/L,1 g/L and 2 5 g/L NaCl+HES solutions for 15 min Results showed all mechanical damaged cells were swelled The cell volume was decreased significantly after exposure to hypertonic hyperoncotic solutions for 15 min, until 7 day later as compared with responding control values at the same time points ( P <0 05) It was suggested that mechanical damaged hippocampal neurons might be unable to regulate their cell volume after exposure to hypertonic hyperoncotic solutions

关 键 词：海马 神经细胞 机械性损伤 细胞容积 HHS 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]