硫普罗宁对急性肝损害保护作用的实验研究  

Study on Protective Effect of Tioproin on D GalN induced Acute Liver Injury in Rats

作　　者：唐望先[1] 虞涤霞 杜荔菁[1] 但自力[1] 张文英[1] 李绍白[1] 

机构地区：[1]同济医科大学附属同济医院肝病研究所,武汉430030 [2]武汉市第七医院肝病科,武汉430071

出　　处：《同济医科大学学报》1999年第4期338-339,349,共3页Acta Universitatis Medicinae Tongji

摘　　要：为了研究硫普罗宁（Ｔｉｏｐｒｏｎｉｎ） 对急性肝损害是否有保护作用， 应用Ｄ－氨基半乳糖（Ｄ－ＧａｌＮ） 造成大鼠急性肝损伤模型。实验分成正常组（Ｎ）、模型组（Ｄ－ＧａｌＮ）、模型＋ 硫普罗宁组（Ｄ－ＧａｌＮ＋ Ｔ）、模型＋ 肝得健组（Ｄ－ＧａｌＮ＋ Ｅ）。观察血清中丙氨酸转氨酶（ＡＬＴ）、肿瘤坏死因子（ＴＮＦ－α）、肝细胞及血浆中脂质过氧化物（ＬＰＯ） 和肝组织及红细胞超氧化物歧化酶（ＳＯＤ） 以及相关的病理变化。结果表明： 模型＋ 硫普罗宁组（Ｄ－ＧａｌＮ＋ Ｔ） 及模型＋ 肝得健组（Ｄ－ＧａｌＮ＋ Ｅ） 血清中ＡＬＴ、ＴＮＦ－α及ＬＰＯ与模型组相比明显下降（Ｐ＜ ０．０１）， 而Ｄ－ＧａｌＮ＋ Ｔ和Ｄ－ＧａｌＮ＋ Ｅ两组的ＳＯＤ活性明显高于模型组（Ｄ－ＧａｌＮ） （Ｐ＜ ０．０１）。提示： 硫普罗宁能降低血清中ＡＬＴ、ＴＮＦ－α的含量及肝组织中氧自由基的产生， 降低血浆和组织中ＬＰＯ水平， 并能提高细胞ＳＯＤ活性， 从而减轻肝损伤。其对肝细胞的保护作用与肝得健一致。The protective effect of Tiopronin(T) on D GalN induced acute liver injury was studied. Thirty five rats were divided into normal (N), model group (D GalN), experimental group (D GalN+T), and experimental control group (D GalN+Essential forte). Serum alanine amainotransferase (ALT), TNF α, LPO in hepatic cells, plasma and SOD in erythrocyte and hepatic tissues were determined. The experimental results showed that serum ALT, TNF α, LPO in hepatic cells and plasma concentrations were markedly decreased in D GalN group ( P <0 01). SOD activity in erythrocyte and heptic tissues was obviously increased in the D GalN+T group and D GalN+E group, as compared with D GalN group ( P <0 01). These findings suggest that Tiopronin ould reduce serum ALT, TNF α contents and the production of oxygen free radical, decrease the LPO level in the hepatic cells and plasma and increase SOD activity in cells, so as to alleviate hepatic injury. The protective effect of Tiopronin is similar to that of essential forte.

关 键 词：硫普罗宁 肝损害 保护作用 实验研究 D-GALN SOD 

分 类 号：R975.5[医药卫生—药品] R575.05[医药卫生—药学]