老年小鼠脾细胞DNA修复能力的变化及gadd45和gadd153基因的可诱导性改变  

The change of DNA repair capacity and gadd45, gadd153 gene inducibility in old mouse splenocytes

作　　者：闫雪冬[1] 张宗玉[1] 童坦君[1] 

机构地区：[1]北京医科大学生物化学与分子生物学系

出　　处：《北京医科大学学报》1999年第4期289-292,共4页Journal of Peking University(Health Sciences)

基　　金：国家自然科学基金

摘　　要：目的：研究小鼠脾细胞ＤＮＡ修复能力的增龄变化以及这种变化的发生与损伤可诱导的ｇａｄｄ４５、ｇａｄｄ１５３基因表达的关联。方法：以紫外线损伤脾细胞ＤＮＡ，用非程序ＤＮＡ合成和单细胞凝胶电泳试验测定ＤＮＡ修复能力；用Ｎｏｒｔｈｅｒｎ杂交方法检测紫外线损伤后ｇａｄｄ４５、ｇａｄｄ１５３基因转录水平的变化。结果：老年小鼠脾细胞ＤＮＡ修复能力低于青年鼠，老年鼠ｇａｄｄ４５和ｇａｄｄ１５３基因的紫外线损伤的可诱导性也低于青年鼠。结论：小鼠脾细胞ＤＮＡ修复能力随增龄而下降，这种变化的发生可能与老年小鼠脾细胞在紫外线损伤后ｇａｄｄ４５、ｇａｄｄ１５３ｍＲＮＡ表达的可诱导性下降有关。Objective: To investigate the change of DNA repair capacity of mouse splenocytes with age and the correlation between this change and the mRNA expression of DNA damage inducible genes gadd45, and gadd153. Methods: The mouse splenocytes were irradiated by UV light, then unscheduled DNA synthesis(UDS) and single cell gel electrophoresis were performed to measure DNA repair capacity, and mRNA expressions of gadd45, and gadd153 were studied by Northern blot analysis. Results: DNA repair capacity of old splenocytes was much lower than that of young ones. After UV irradiation, the mRNA expressions of gadd45 and gadd153 increased obviously in both the young and the old splenocytes. But the increase was significantly higher in the young than in the old. Conclusion: There is a decline in DNA repair capability in mouse splenocytes with age. This may be partly due to a relatively lower mRNA expressons of gadd45, and gadd153 genes in old splenocytes after UV irradiation.

关 键 词：脾细胞 DNA修复 gadd45基因 gadd153基因 表达 

分 类 号：Q756[生物学—分子生物学] Q523