同型半胱氨酸对大鼠心肌细胞钙摄取的影响  

Effect of homocysteine on calcium uptake in rat cardiomyocyte

作　　者：李载权[1,2,3] 马健[1,2,3] 张钧华[1,2,3] 高炜 周爱儒[1,2,3] 唐朝枢 刘乃奎[1,2,3] 

机构地区：[1]北京医科大学第一医院心血管研究所 [2]中国人民解放军海军总医院心内科 [3]北京医科大学心血管基础研究所

出　　处：《北京医科大学学报》1999年第4期300-301,305,共3页Journal of Peking University(Health Sciences)

基　　金：国家自然科学基金

摘　　要：目的：研究同型半胱氨酸（ｈｏｍｏｃｙｓｔｅｉｎｅ，ＨＣＹ）对心肌细胞钙摄取的影响及其作用机制。方法：采用同位素技术测定培养大鼠心肌细胞钙的摄取，观察不同ＨＣＹ浓度对培养心肌细胞钙摄取的影响及蛋白激酶Ｃ（ｐｒｏｔｅｉｎｋｉｎａｓｅＣ，ＰＫＣ）抑制剂或蛋白磷酸酶抑制剂与ＨＣＹ同时培养对培养心肌细胞钙摄取的影响。结果：ＨＣＹ呈浓度依赖性促进心肌细胞钙的摄取，ＨＣＹ促进心肌细胞钙摄取的作用为ＰＫＣ抑制剂Ｈ７和多粘菌素（ｐｏｌｙｍｙｃｉｎＢ，ＰＢ）所抑制，并且受蛋白磷酸酶抑制剂Ｏｋａｄａｉｃａｃｉｄ作用而增强。结论：ＨＣＹ促进心肌细胞钙摄取，可能与ＰＫＣ所介导的蛋白磷酸化过程有关。Objective: To study the effect of homocysteine (HCY) on calcium uptake in rat cardiomyocyte and its mechanism. Methods: The calcium uptake by rat cardiomyocyte was assayed with isotopic methods. The in vitro study observed the influences of liner increasing concentrations of HCY, and those of protein kinase C (PKC) inhibitors and selective inhibitor of protein phosphatase type 1 and 2A, Okadaic acid, combined with HCY on the calcium uptake in rat cardiomyocyte. Results: HCY promoted the calcium uptake in a dose dependent way, and this promoting effect was blocked by PKC inhibitors, H7 and polymycin B, but stimulated by selective inhibitor of protein phosphatase type 1 and 2A, Okadaic acid. Conclusion:The calcium uptake in rat cardiomyocytes was induced by HCY possibly through cellular PKC pathway.

关 键 词：半胱氨酸 药理学 心肌细胞 钙代谢 

分 类 号：R977.4[医药卫生—药品]