小鼠凋亡相关新基因TFAR19 cDNA的克隆化和序列分析  

The cDNA cloning and sequencing of a novel mouse apoptosis related gene TFAR19

作　　者：宋泉声[1] 韩文玲[1] 刘红涛[1] 马大龙[1] 

机构地区：[1]北京医科大学免疫学系

出　　处：《北京医科大学学报》1999年第4期309-311,共3页Journal of Peking University(Health Sciences)

摘　　要：目的：了解一个细胞凋亡相关新基因ＴＦＡＲ１９在不同种属间的序列同源性。方法：利用表达序列标签（ｅｘｐｒｅｓｅｄｓｅｑｕｅｎｃｅｔａｇ，ＥＳＴ）拼接技术、ＲＴＰＣＲ、ＤＮＡ序列测定技术及计算机分析技术。结果：首次成功进行了小鼠ＴＦＡＲ１９ｃＤＮＡ编码区的ｃＤＮＡ克隆化和序列分析，发现小鼠和人ＴＦＡＲ１９在核苷酸水平上有８１．４％的同源性，在氨基酸水平上有高达９６％的同源性。功能区分析发现，小鼠ＴＦＡＲ１９ｃＤＮＡ序列含编码１２６个氨基酸的开放性读码框架，含１个可能的ｃＡＭＰ和ｃＧＭＰ依赖的蛋白激酶磷酸化位点，４个可能的ＰＫＣ磷酸化位点。其Ｃ端的“ＥＤＤＡＤＹ”序列与人ＤＮＡ拓扑异构酶Ｉ１４１１４７位残基序列完全相同。结论：小鼠ＴＦＡＲ１９是与人ＴＦＡＲ１９高度同源的新基因，与ＤＮＡ拓扑异构酶Ｉ可能有功能相关性。Objective: To find out the sequence homology of a novel gene TFAR19 (TF 1 cell apoptosis related gene 19) between different species. Methods: The EST (expressed sequence tag) sequence alignment method, RT PCR, DNA sequencing, and computer analysis were used for the cDNA cloning. Results: The coding region of mouse TFAR19 cDNA was successfully cloned and sequenced. It was found that there was 81.4% homology at the nucleotide level and 96% homology at the amino acid level between human and mouse TFAR19 cDNA. The sequence revealed an open reading frame encoding 126 amino acids, one potential cAMP and cGMP dependent and four potential protein kinase C phosphorylation sites. A motif “EDDADY” localized at its C terminal end was the same as the residues 141 147 of human DNA topoisomerase I. Conclusion: The mouse TFAR19 sequence is highly homologous with human TFAR19 and may have functional relationship with DNA topoisomerase I.

关 键 词：TFAR19 CDNA 细胞凋亡 序列分析 分子克隆 

分 类 号：Q343.11[生物学—遗传学]