照射与非照射方法诱导小鼠皮肤移植耐受  

Transplantation tolerance to mice skin allografts induced with irradiate and non irradiate protocols

作　　者：苑学礼[1] 宋娴媛 谢蜀生[1] 郭应禄[1] 

机构地区：[1]北京医科大学第一医院泌尿外科

出　　处：《北京医科大学学报》1999年第4期318-320,共3页Journal of Peking University(Health Sciences)

基　　金：国家自然科学基金

摘　　要：目的：比较不同方法诱导移植耐受的效果，探讨有临床应用前景的非照射耐受诱导方法。方法：分别以主要组织相容性抗原（ｍａｊｏｒｈｉｓｔｏｃｏｍｐａｔｉｂｉｌｉｔｙｃｏｍｐｌｅｘ，ＭＨＣ）完全不同的雄性ＢＡＬＢ／ｃ和雌性Ｃ５７ＢＬ／６小鼠作为皮肤移植的供体和受体，移植前对受体小鼠单独或联合应用５Ｇｙ全身照射、０．１ｍｌ抗淋巴细胞血清注射、３×１０７供体骨髓细胞静脉输注、每千克体重１５０ｍｇ环磷酰胺腹腔注射。结果：各实验组的皮肤移植物存活时间均较对照组明显延长，混合淋巴细胞反应和迟发型超敏反应也表现为供体特异性降低。移植前应用抗淋巴细胞血清、骨髓输注和皮肤移植后注射环磷酰胺可以延长移植物存活时间，平均存活２３．６ｄ，取得与全身照射后输入供体骨髓相似的耐受诱导效果（平均２０．４ｄ）。结论：采用比较温和的非照射的预处理方法，在不应用免疫抑制剂的情况下，也可获得一定程度的免疫耐受，达到延长移植物存活的目的。Objective: To establish a transplantation tolerance induction protocol which might be applied in clinical situation in the future. Methods: Fully MHC mismatched male BALB/c (H 2 d) and female C57BL/6 (B6, H 2 b) mice were used as skin transplantation donors and recipients, respectively. Before operation B6 mice were conditioned with 5 Gy whole body irradiation (WBI), 0.1 ml ALS (antilymphocytic serum)injection for three times, 3×10 7 donor bone marrow cells transfusion (BMT), 150 mg·kg -1 cyclophosphamide(CP) separately or combinatively. Results: When ALS was used combinatively with BMT and CP, the allograft survival was significantly prolonged, mean survival time was 23.6 days, just as the result of WBI+BMT induction. Conclusion: Transplantation tolerance can also be induced by non irradiate pretreatment.

关 键 词：皮肤移植 免疫耐受 移植物存活 辐射效应 

分 类 号：R622.1[医药卫生—整形外科]