欧山羊草高分子谷蛋白亚基向小麦的转入及其遗传规律  

Transferred HMW Glutenin Subunits from Aegilops biuncialis to Common Wheat and Its Genetic Analysis

作　　者：孙彬[1] 周建平[1] 杨足君[1] 李光蓉[1] 文正南[1] 陈明静[2] 任正隆[1,3] 

机构地区：[1]电子科技大学生命科学与技术学院,成都610054 [2]四川达州市农业科学研究所,四川达州635000 [3]四川农业大学植物遗传育种省级重点实验室,四川雅安625014

出　　处：《中国农学通报》2011年第1期42-44,共3页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目"兼抗性小麦-欧山羊草新种质的创制;鉴定及抗病基因的利用"(30800685);"小麦开花后叶片抗衰老的生理和遗传学基础研究"(30730065)

摘　　要：欧山羊草具有两个容易用聚丙烯酰胺凝胶电泳与小麦区别的高分子谷蛋白亚基,分别命名为Glu-Aeb1和Glu-Aeb2。采用聚丙烯酰胺凝胶电泳(SDS-PAGE)方法对21份欧山羊草与小麦杂交后代BC2F6和15份BC2F5与小麦杂交F0种子的谷蛋白亚基组成进行了鉴定。实验结果表明,36份材料全部都有欧山羊草高分子谷蛋白亚基的转入。Glu-Aeb1在BC2F6和杂交组合F0中的传递频率分别为59.52%和20.94%。而Glu-Aeb2在BC2F6和杂交组合F0中的传递频率分别为40.48%和22.96%。Two HMW glutenin subunits in Aegilops biuncialis, named with Glu-Aeb1 and Glu-Aeb2,can be easily identified from common wheat using polyacrylamide gel electrophoresis. 21 Aegilops biuncialis and common wheat hybrid progenies （ BC 2 F 6 ） along with 15 hybrids （ F 0 ） of BC 2 F 5 and common wheat hybrid progenies were studied using SDS-PAGE. The results showed that all the materials are in possession of Aegilops biuncialis HMW glutenin subunits. The transfer frequency of Glu-Aeb1 in BC 2 F 6 and F 0 are 59.52% and 20.94%,respectively. While the transfer frequency of Glu-Aeb2 in BC 2 F 6 and F 0 are 40.48% and 22.96%, respectively.

关 键 词：欧山羊草 小麦 Glu-Aeb1 Glu-Aeb2 聚丙烯酰胺凝胶电泳 

分 类 号：S512.1[农业科学—作物学] S336