IP_3敏感的钙离子通透性通道参与茉莉酸诱导的钙动员  

IP_3 Sensitive Calcium Channel Involved in the Jasmonic Acid Induced Calcium Mobilization

作　　者：李洋洋[1,2] 杜希华[1] 于涌鲲[2] 赵福宽[2] 孙清鹏[2] 

机构地区：[1]山东师范大学生命科学学院,济南250014 [2]北京农学院生物技术学院,北京102206

出　　处：《中国农学通报》2011年第1期149-153,共5页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金"环核苷酸门控的离子通道在拟南芥茉莉酸信号通路中的作用";"AtCNGC2/AtCNGC19在茉莉酸诱导的钙离子动员中的分子机制"(30700428;30911130166);北京市自然科学基金"AtCNGC离子通道在茉莉酸诱导的钙动员中的作用"(5072009);北京市科技新星计划"AtCNGC2或AtCNGC4在茉莉酸诱导的钙离子动员中的作用"(2006B26)

摘　　要：以低温导入法将钙离子荧光探针Fluo-3/AM导入拟南芥叶片细胞中,利用LAS AF(LeicaApplication Suite-Advanced Fluorescence)软件记录肝素对茉莉酸(JA)诱导的胞内钙离子荧光强度的变化。结果显示,经不同浓度的肝素预处理后,拟南芥叶细胞中胞内钙离子的荧光强度降低,再用100μmol/L JA处理时,其荧光强度升高,但仅与未经肝素处理的荧光强度相当。实验证明,肝素预处理可抑制JA诱导的胞内钙离子浓度的升高。Arabidopsis thaliana leaves were labeled by fluorescent probe Fluo-3/AM under low temperature at 4℃ to measure the fluorescent intensity of intracellular Ca 2 ＋ which was pretreated with heparin on jasmonic acid （JA）-induced. The results showed that the fluorescent intensity of [Ca 2 ＋ ] cyt was reduced after pretreated with different concentration of heparin, and then treated with 100 μmol/L JA, the fluorescent intensity of [Ca 2 ＋ ] cyt was close to the fluorescent intensity which was not pretreated with heparin. The experiment showed that the pretreatment with heparin could inhibit the increase of the intracellular Ca 2＋ concentration significantly which JA-induced in leaves of Arabidopsis thaliana.

关 键 词：拟南芥 钙离子 肝素 茉莉酸 

分 类 号：Q291[生物学—细胞生物学]