SB-431542对梅花鹿鹿茸软骨层细胞增殖的影响  

Effects of SB-431542 on the Proliferation of Sika Deer Antler Chondrocytes

作　　者：韩玉帅[1] 张璐[1] 赵丽红[1] 郭斌[1] 冯海华[1] 岳占碰[1] 

机构地区：[1]吉林大学畜牧兽医学院,长春130062

出　　处：《中国农学通报》2011年第1期362-365,共4页Chinese Agricultural Science Bulletin

基　　金：国家高技术研究发展计划资助项目"梅花鹿鹿茸再生相关基因的筛选;克隆及功能分析"(2007AA10Z150);国家自然科学基金资助项目"PTHrP-IHH信号通路对梅花鹿茸角再生的调控"(31072099);长春市科技支撑计划资助项目"优质梅花鹿良种选育与高效快繁关键技术研究与开发"(08KZ36);教育部新教师基金资助项目"梅花鹿茸角ColX基因表达分布及调控分析"(200801831048);高等学校博士学科点专项科研基金资助项目"甲状旁腺素相关肽及其受体在梅花鹿茸角内的表达及与茸角再生的关系"(20100061110078)

摘　　要：通过研究转化生长因子β(transforming growth factor-β,TGF-β)的特异性小分子拮抗剂SB-431542对梅花鹿鹿茸软骨层细胞增殖的影响,探讨转化生长因子β在鹿茸快速生长中的调节机制。分离培养生长30天的梅花鹿鹿茸软骨层细胞,将传代培养第2代的细胞经不同浓度的SB-431542(0、1、3、5、8、10μmol/L)作用,培养48h后用MTT法测其细胞增殖的变化,并用SPSS软件分析其差异性。结果显示,SB-431542处理组细胞增殖活性都低于对照组(P<0.05),其中浓度为8μmol/L和10μmol/L的SB-431542处理组与对照组相比差异非常显著(P<0.01),随着添加SB-431542浓度的升高,对细胞的生长速度抑制更加明显,试验结果表明,TGF-β对维持梅花鹿鹿茸软骨层细胞的快速增殖有重要作用。The regulatory mechanism of TGF-β on the rapid growth of antler was investigated by studying the effect of specific micro-molecule antagonist SB-431542 of transforming growth factor- β （TGF-β） on the cell proliferation of sika antler chondrocytes. The sika deer antler chondrocytes were isolated at the 30th day of the growth, then the second generation cells were incubated with 0, 1, 3, 5, 8, 10 μmol/L of SB-431542, after 48 hours of culture, the MTT assay was used to examine the proliferation of antler chondrocytes and the SPSS software was used to analyze the difference of the results. The result showed that the proliferation activity of cells treated with SB-431542 was lower than that of the control group （P0.05）, among them, the proliferation activity of cells treated with SB-431542 at the concentration of 8 μmol/L and 10 μmol/L was significantly lower than that of the control （P0.01）, with the increased concentration of SB-431542, the inhibition to cell proliferation rate was more significant. In conclusion, these results indicated that TGF-β might play an important role to maintain the rapid proliferation of sika antler chondrocytes.

关 键 词：SB-431542 梅花鹿鹿茸 软骨层细胞 增殖 

分 类 号：S8[农业科学—畜牧兽医] Q24[生物学—细胞生物学]